前沿科研成果融入分析化学实验教学——高亮度荧光聚合物纳米颗粒的合成及表征

Integrating Frontier Scientific Research into Analytical Chemistry Experiment Teaching:Synthesis and Characterization of High-Brightness Fluorescent Polymer Nanoparticles

荧光纳米颗粒在生物成像、传感和诊断等领域展现了广阔的应用前景。然而,传统的有机染料常受到聚集导致荧光猝灭效应的限制。本文以自组装聚合物纳米颗粒的制备和表征为例,将反离子增强荧光策略引入分析化学实验教学,为学生提供了一种新型荧光增强方法的实践体验。通过这一实验,学生能够掌握聚合物纳米颗粒的合成、表征技术及反离子增强荧光发射的原理,深入理解荧光聚集导致猝灭效应及其解决方法。该实验设计不仅有效提升了学生对分析化学知识的理解和应用能力,还拓展了其对纳米材料科学的认知,为未来在相关领域的研究奠定了坚实基础。

Fluorescent nanoparticles hold immense potential in various applications,including bioimaging,sensing,and diagnostics.However,traditional organic dyes often suffer from aggregation-caused quenching(ACQ)effects.This paper introduces an innovative approach to enhance fluorescence by incorporating the counterion-enhanced strategy into analytical chemistry experiment teaching,exemplified by the synthesis and characterization of self-assembled polymer nanoparticles.This experiment provides students with practical experience in a novel fluorescence enhancement method.Through this hands-on experience,students can master the techniques for synthesizing and characterizing polymer nanoparticles,understand the principle of counterion-enhanced fluorescence,and gain a deeper insight into the ACQ effect and its solutions.This experimental design not only effectively improves students'understanding and application of analytical chemistry knowledge but also broadens their knowledge of nanomaterial science,laying a solid foundation for future research in relevant fields.