离子色谱法检测短链脂肪酸的研究进展

Research Progress on Detection of Short-Chain Fatty Acids by Ion Chromatography

离子色谱法是近年快速发展的检测技术,具有高灵敏度、高选择性、样品用量少等优点.短链脂肪酸作为肠道菌群的代谢产物,对维持机体生理健康具有重要作用.以“离子色谱法”“短链脂肪酸”“样品前处理”等为检索词,检索中国知网、维普文献数据库和PubMed数据库,归纳、分析从2007年到2023年的文献,对以离子色谱法为主的短链脂肪酸前处理方式和检测方法进行总结和归纳.结果发现,短链脂肪酸的前处理方法主要有萃取法、离心法、柱前衍生化法等,而离子色谱法中大多采用离心法或简单萃取法.在流动相中加入适量盐酸酸化有助于增加短链脂肪酸的保留率,加入有机改性试剂能够改善短链脂肪酸的峰形和分离度.离子色谱法结合在线富集或复杂基质消除方法,能够实现痕量短链脂肪酸的准确定量分析.离子色谱法与质谱联用能够满足复杂基质样品的检测要求,是未来发展的趋势.

Ion chromatography is a rapidly developing detection technology in recent years.It has the advantages of high sensitivity,high selectivity and low sample consumption.As gut microbial metabolites,short-chain fatty acids play a crucial role in maintaining physiological health.Using"ion chromatography","short-chain fatty acids","sample pretreatment"as search terms,the CNKI,VIP database and PubMed database were searched,summarized and analyzed from 2007 to 2023.The pretreatment and detection methods of short-chain fatty acids using ion chromatography were summarized and generalized.The results showed that the main pretreatment methods of short-chain fatty acids included extraction,centrifugation,and pre-column derivatization,among others.Ion chromatography mostly used centrifugation and extraction.Adding an appropriate amount of hydrochloric acid to the mobile phase can enhance the retention rate of short-chain fatty acids,and the addition of organic modification reagents can improve the peak shape and resolution.Ion chromatography combined with on-line enrichment or complex matrix elimination can achieve accurate quantitative analysis of trace short-chain fatty acids.The combination of ion chromatography and mass spectrometry can meet the detection requirements of complex matrix samples,which is a trend of future development.