肺、肾组织透明化及其深度成像的探索

Exploration of Tissue Clearing and Deep Imaging of Lung and Kidney

传统获取生物组织样本内部信息都需要借助机械切片来实现,耗时耗力且容易造成样品损坏丢失信息.组织透明化技术和显微成像技术的发展实现了无需机械切片就可以观察到组织乃至整个生命体内部结构.利用CUBIC(clear,unobstructed brain imaging cocktails and computational analysis)方法实现了小鼠肺和肾组织透明化,并比较了其在Olympus转盘共聚焦显微镜和Luxendo光片显微镜的成像效果,为肺和肾组织样品透明化条件和成像设备选择及使用提供了参考.此外,结合水凝胶型膨胀试剂对较为质密的肾组织膨胀透明化进行了探索,为透明化组织的高分辨成像提供了参考.

Traditionally,the internal information of biological tissue samples needs to be obtained by mechanical slicing,which is time-consuming,labor-intensive,easy to cause sample damage and loss of information.The development of tissue clearing and microscopic imaging technology realizes the observation of the internal structure of tissue and even whole body without mechanical slicing.The tissue clearing of lung and kidney of mice were obtained using CUBIC(clear,unobstructed brain imaging cocktails and computational analysis)method,and the imaging effect of the tissue on Olympus spinning-disk confocal microscope and Luxendo light-sheet microscope were compared,which provides a reference for the conditions of lung and kidney tissue clearing and the selection and application of imaging equipment.In addition,tissue expansion and tissue clearing of relatively dense kidney tissue was explored in combination with tissue expansion gel,which provides a reference for high resolution imaging of cleared tissue.