一株丁酸梭菌冻干菌粉的获得及体内安全性评价

Preparation of a Freeze-dried Powder of Clostridium butyricum and Its in vivo Safety Evaluation

该试验对一株丁酸梭菌BLCC1-0022利用生物发酵罐扩大培养获得丁酸梭菌冻干菌粉,并利用小鼠经口急性毒性试验评价其体内的毒性反应。结果表明:丁酸梭菌BLCC1-0022经500 L生物发酵罐扩大培养,在稳定期18 h时停止发酵,pH降至最低为5.15,发酵液活菌数2.89×10^(8) CFU/mL,芽孢率96%,全部发酵液经过冻干得到冻干菌粉活菌数4.1×10^(10) CFU/g。与对照组相比,灌胃丁酸梭菌低剂量组(5×10^(8) CFU/只)、中剂量组(5×10^(9)和5×10^(10) CFU/只)、高剂量组(5×10^(11) CFU/只)小鼠的生长性能、血液指标均无显著差异(P>0.05),不会发生细菌移位现象,未对小鼠的心脏、肝脏和脾脏组织造成病理损伤。总之,丁酸梭菌BLCC1-0022不会对小鼠内脏、血液及生长性能产生毒性反应,体内安全性较高。

This experiment involved scaling up the cultivation of Clostridium butyricum BLCC1-0022 in a bioreactor to obtain a freeze-dried powder of Clostridium butyricum,followed by an assessment of its toxicity in vivo through acute oral toxicity testing in mice.The results showed that Clostridium butyricum BLCC1-0022,when cultured in a 500 L bioreactor,reached the stationary phases at 18 hours with a minimum pH of 5.15.The viable cell count in the fermentation broth was 2.89×10^(8) CFU/mL,with a spore yield of 96%.The freeze-dried bacterial powder obtained from the entire fermentation broth had a viable bacterial count of 4.1×10^(10) CFU/mL(CFU/g).Compared to the control group,the low dose(5×10^(8) CF U),medium dose(5×10^(9) and 5×10^(10) CFU),and high-dose(5×10^(11) CFU)groups of mice administered Clostridium butyricum showed no significant differences in growth performance and blood parameters(P>0.05).Furthermore,no bacterial translocation occurred,and there was no pathological damage to the heart,liver,or spleen tissues of the mice.In conclusion,Clostridium butyricum BLCC1-0022 did not induce toxic reactions in the internal organs,blood,or growth performance of the mice,indicating a high level of in vivo safety.