槲皮素对山羊精液低温保存的影响

Effect of Quercetin on the Cryopreservation of Goat Semen

山羊精液在储存过程中容易受到氧化应激的损害。槲皮素是一种广泛存在于植物中的黄酮醇化合物,具有多种生物活性,已被证明可以改善精子质量。该试验旨在研究精液稀释液中添加槲皮素对低温(4℃)储存山羊精液质量的影响。采集9只健康1~3岁陕西关中奶山羊的精液,添加不同浓度(10μmol/L、25μmol/L、50μmol/L、100μmol/L)槲皮素后分析低温保存山羊精液的抗氧化性、ATP含量及相关磷酸化蛋白表达水平。结果表明:添加槲皮素可提高低温保存精子的活力,保护质膜和顶体的完整性,抑制精子蛋白的去磷酸化,提高磷酸化蛋白的表达水平;槲皮素可通过cAMP-PKA信号通路抑制精液中过多活性氧(reactive oxygen species,ROS)诱导的精子蛋白去磷酸化,且槲皮素的最佳浓度为50μmol/L。因此,在山羊精液低温保存时添加50μmol/L的槲皮素可以改善精液质量。

Goat semen is susceptible to damage from oxidative stress during storage.Quercetin,a flavonol compound widely found in plants,has several biological activities and has been shown to improve sperm quality.The aim of this experiment was to investigate the effect of quercetin addition to semen dilutions on semen quality of goats stored at low temperature(4°C).In this experiment,semen was collected from 1 to 3-year-old healthy Shaanxi Guanzhong dairy goats(n=9),and the antioxidant property,ATP content and related phosphorylated proteins of cryopreserved goat semen were analyzed after the addition of quercetin at different concentrations(10μmol/L,25μmol/L,50μmol/L and 100μmol/L).The results showed that the addition of quercetin could improve the viability of cryopreserved spermatozoa,protect the integrity of the plasma membrane and acrosome,inhibit the dephosphorylation of sperm proteins,and increase the expression level of phosphorylated proteins;the addition of quercetin could inhibit the dephosphorylation of spermatozoa proteins induced by excessive reactive oxygen species(ROS)in semen through the cAMP-PKA signaling pathway.The optimal concentration of quercetin is 50μmol/L.Namely,the addition of 50μmol/L quercetin to goat semen during cryopreservation can improve semen quality.