水稻雄性不育系裂颖的生理变化及转录组分析

Physiological Changes and Transcriptome Analysis of Glume-gapping in Rice Male Sterile Lines

为探究水稻雄性不育系裂颖的发生机理,挖掘水稻裂颖发生的关键基因,以期减少杂交水稻制种过程中裂颖的发生,以裂颖性状差异较大的雄性不育系香玉5A、玥020S及其恢复系R3576为供试材料,采集香玉5A和玥020S开花期颖果进行转录组测序分析,并测定了颖花开闭相关生理指标。结果显示,重度裂颖品种香玉5A颖果中可溶性糖和茉莉酸含量均高于轻度裂颖品种玥020S,而生长素含量则低于玥020S。转录组分析获得3099个差异表达基因(DEGs);GO富集分析表明,这些差异表达基因主要在代谢和催化过程中发挥作用;KEGG Pathway分析表明,这些差异表达基因主要通过植物病原互作、植物激素信号转导、淀粉与蔗糖代谢等途径富集。从淀粉与蔗糖代谢途径、植物激素信号转导途径和转录因子中筛选出了11个可能与裂颖发生密切相关的基因,结果表明Os07g0543300、OsJAR1和OsMADS16等基因可能是导致水稻雄性不育系裂颖发生的关键基因。本研究获得了不同裂颖性状水稻雄性不育系的差异表达基因及其功能注释信息,为进一步探明水稻不育系裂颖发生的分子机制奠定了基础。

To explore the mechanism of glume-gapping in rice male sterile lines and identify the key genes of glumegapping in rice,hoping to reduce the occurrence of glume-gapping in hybrid rice seed production,the male sterile lines Xiangyu 5A(XY5A)and Yue 020S(Y020S)with significant differences in glume-gapping traits,and their restorer line R3576 were used as experimental materials.The glumes of XY5A and Y020S at flowering stage were collected for transcriptome sequencing analysis,and the physiological indexes related to glume opening and closing were determined.The results showed that the contents of soluble sugar and jasmonic acid in XY5A with heavily glumegapping were higher than those of Y020S with lightly glume-gapping,while the content of auxin was exactly the opposite.Transcriptome analysis obtained 3099 differentially expressed genes(DEGs).GO enrichment analysis showed that these DEGs mainly play a role in metabolic and catalytic processes.KEGG Pathway analysis showed that these DEGs were mainly enriched through plant-pathogen interaction,phytohormone signal transduction,starch and sucrose metabolism.Eleven genes that might be closely related to glume-gapping occurrence were screened from starch and sucrose metabolic pathways,phytohormone signal transduction pathways and transcription factors.The results indicated that the genes such as Os07g0543300,OsJAR1 and OsMADS16 might be the key genes leading to glume-gapping in rice male sterile lines.In this study,the differentially expressed genes and their functional annotation information of rice male sterile lines with different glume-gapping traits were obtained,laying a foundation for further exploring the molecular mechanism of glume-gapping in rice male sterile lines.