血清IL-35 CCL19水平与系统性红斑狼疮患儿免疫学指标的相关性研究

Correlation between Serum IL-35 and CCL19 Levels and Immunological Indexes in Children with Systemic Lupus Erythematosus

目的:观察系统性红斑狼疮(SLE)患儿血清白介素-35(IL-35)、C-C基序趋化因子配体19(CCL19)变化情况,并分析其与患儿免疫学指标的关联性。方法:纳入2022年1月至2024年3月本院收治的SLE患儿97例,并同期选取97例健康儿童进行对照研究,观察患儿血清IL-35、CCL19水平变化,并分析其与免疫学指标(包括抗ds-DNA抗体、免疫球蛋白(Ig)及补体C3、C4)的相关性。结果:与健康儿童相比,SLE患儿血清IL-35水平明显较低,CCL19水平明显较高,差异均有统计学意义(P<0.05)。与非活动期SLE患儿相比,活动期SLE患儿血清IL-35、C3、C4水平明显较低,CCL19、抗ds-DNA抗体、IgM、IgG、IgA水平明显较高,差异均有统计学意义(P<0.05)。Pearson相关性分析显示:SLE患儿血清IL-35水平与抗ds-DNA抗体、IgM、IgG、IgA水平成负相关性,与C3、C4水平成正相关,差异均有统计学意义(r=-0.314、-0.203、-0.301、-0.201、0.318、0.383,P<0.05);SLE患儿血清CCL19水平与抗ds-DNA抗体、IgM、IgG、IgA水平成正相关性,与C3、C4水平成负相关差异均有统计学意义(r=0.272、0.209、0.362、0.202、-0.219、-0.391,P<0.05)。线性回归显示:SLE患儿抗ds-DNA抗体与IL-35水平存在负向影响关系,差异有统计学意义(P<0.05),C3则与IL-35水平存在正向影响关系,差异有统计学意义(P<0.05);SLE患儿IgG与CCL19水平存在正向影响关系,差异有统计学意义(P<0.05)。结论:SLE患儿血清IL-35、CCL19水平异常变化,与抗ds-DNA抗体、IgM、IgG、IgA水平存在一定的相关性,其中抗ds-DNA抗体、C3与IL-35,IgG与CCL19间存在一定的影响关系。

Objective:To observe the changes of serum interleukin-35(IL-35)and C-C motif chemokine ligand 19(CCL19)in children with systemic lupus erythematosus(SLE),and to analyze the association with immunological indexes.Methods:A total of 97 children with SLE in the hospital were included from January 2022 to March 2024,and 97 healthy children were selected for control study during the same time period.The changes of serum IL-35 and CCL19 levels were observed,and the correlation with immunological indexes[including anti-ds-DNA antibody,immunoglobulin(Ig),complements C3 and C4]was analyzed.Results:Compared with the healthy children,the children with SLE had a significantly lower serum IL-35 level and a significantly higher serum CCL19 level(P<0.05).Compared with the children with inactive SLE,serum levels of IL-35,C3,and C4 in the children with active SLE were significantly lower while serum levels of CCL19,anti-ds-DNA antibody,IgM,IgG,and IgA were significantly higher(P<0.05).Pearson correlation analysis showed that the serum level of IL-35 in children with SLE was negatively correlated with the levels of anti-ds-DNA antibody,IgM,IgG,and IgA,and was positively correlated with the levels of C3 and C4(P<0.05).Serum CCL19 level was positively correlated with the levels of anti-ds-DNA antibody,IgM,IgG and IgA,and was negatively correlated with the levels of C3 and C4 in children with SLE(r=-0.314,-0.203,-0.301,-0.201,0.318,0.383,P<0.05).Linear regression analysis suggested that anti-ds-DNA antibody had a negative impact on IL-35 level in children with SLE(r=0.272,0.209,0.362,0.202,-0.219,-0.391,P<0.05)while C3 had a positive impact on IL-35 level(P<0.05).IgG in children with SLE had a positive impact on CCL19 level(P<0.05).Conclusion:The abnormal changes of serum IL-35 and CCL19 levels in children with SLE are correlated with the levels of anti-ds-DNA antibody,IgM,IgG and IgA.There is a certain influence relationship between anti-ds-DNA antibody,C3 and IL-35,IgG and CCL19.