基于SSR分子标记的晋北地区引进马铃薯种质资源遗传多样性分析

Genetic Diversity Analysis of Introduced Potato Germplasm Resources in Northern Shanxi Province Based on SSR Molecular Markers

为揭示马铃薯(Solanum tuberosum L.)品种的遗传变异特征,对我国61份种质材料进行遗传多样性分析。结果表明,12对引物共扩增出100个多态性条带,多态位点达94.17%,检测出75个等位基因位点,引物的平均多态信息量为0.5240,具有较高的多态性。61份种质材料个体间平均有效等位基因数为2.6306,平均香农多样性Shannon指数为1.1413,表明供试马铃薯品种遗传多样性较为丰富。基于Structure结构分析可以将61份马铃薯材料大致分为2大类群,主坐标分析和聚类分析进一步将参试品种归为5类,分组结果具有一定相似性。

A genetic diversity study was carried out on 61 germplasms in China in order to examiner the genetic variation characteristics of potato(Solanum tuberosum L.)germplasm materials.The outcomes demonstrated that 12 pairs of primers amplified 100 polymorphic bands in total,yielding a polymorphism rate of 94.17%.Twelve sets of primers identified 75 allelic loci;the average polymorphic information of the primers was 0.5240,indicating substantial polymorphism.The average number of effective alleles between individuals in 61 germplasm materials was 2.6306 and the average Shannon index was 1.1413,suggesting a relatively significant genetic diversity among the examined potato cultivars.The 61 potato materials were roughly classified into two groups based on structure analysis.Principal coordinate analysis and cluster analysis further classified the tested varieties into 5 groups,and these grouping results have certain similarities.These results would provide theoretical reference for the utilization of elite potato germplasm resources,genetic variation,and scientific breeding.