超声和微波原理快速石蜡技术的应用比较

Comparison of the fast paraffin technology of ultrasound and microwave principles

目的比较基于超声与微波2种不同原理快速石蜡技术的应用效果。方法收集2022年1-12月苏州大学附属第一医院病理科通过超声空化法与微波法处理的快速石蜡标本,通过分析苏木精-伊红(HE)染色制片优良率、免疫组化染色、荧光原位杂交技术(FISH)检测效果及处理时间、试剂环保性等,比较2种不同原理快速石蜡技术的应用效果。结果超声空化法共处理快速石蜡标本1989例,微波法处理快速石蜡标本1540例,2种技术均广泛应用于全身多处小组织活检,HE切片优良率均达到99%,2种技术处理的快速石蜡切片HE染色评分比较,差异无统计学意义(P>0.05)。超声空化法处理的快速石蜡标本有3例出现脱片现象(脱片率为0.15%),微波法处理的快速石蜡标本8例出现脱片现象(脱片率为0.53%),2组脱片率比较,差异无统计学意义(P>0.05);2种技术免疫组化染色效果均满意;2种技术处理的快速石蜡切片FISH检测效果满意,均不影响结果判读;超声空化快速石蜡技术处理时间为69(63,76)min,微波法快速石蜡技术处理时间为123(119,126)min,2组比较,差异有统计学意义(P<0.05)。结论2种不同原理快速石蜡技术制片优良率均高于99%,在HE染色、免疫组化及FISH检测方面效果一致,但综合组织脱片率及组织处理时间考虑,基于超声空化原理的快速石蜡技术更值得临床推广。

Objective To compare the application effects of rapid paraffin techniques based on two different principles:ultrasound and microwave.Methods Rapid paraffin samples processed by ultrasonication and microwave methods from January to December 2022 at the Department of Pathology,the First Affiliated Hospital of Soochow University were collected.The application effects of these two techniques were compared by analyzing the excellent rate of Hematoxylin and Eosin(HE)staining,immunohistochemical staining,fluorescence in situ hybridization(FISH)outcomes,processing time,and reagent environmental friendliness.Results A total of 1989 rapid paraffin samples were processed using ultrasonication,and 1540 samples were processed using the microwave method.Both techniques were extensively applied to small tissue biopsies from various parts of the body,with an excellent HE slide quality rate reaching 99%.There was no statistically significant difference in HE staining scores between the two techniques(P>0.05).Three instances of slide detachment were observed in samples processed by ultrasonication(detachment rate of 0.15%),compared to eight in the microwave-processed samples(detachment rate of 0.53%);however,the difference was not statistically significant(P>0.05).Both techniques provided satisfactory results in immunohistochemical staining;the FISH detection outcomes of rapid paraffin slices processed by both techniques were satisfactory and did not affect result interpretation.The processing time for ultrasonication rapid paraffin technique was 69(63,76)minutes,whereas it was 123(119,126)minutes for the microwave technique,showing a statistically significant difference(P<0.05).Conclusion Both rapid paraffin techniques produced an excellent slide quality rate of over 99%,and consistent results in HE staining,immunohistochemistry,and FISH detection.However,considering the overall detachment rate and tissue processing time,the ultrasonication-based rapid paraffin technique is more deserving of clinical promotion.