富氢水对加州鲈幼苗运输应激的调节作用研究

Research on the Effects of Hydrogenenriched Water on Transport Stress in Juvenile Largemouth Bass,Micropterus Salmoides

为探究富氢水对加州鲈幼苗运输应激的调节作用。该研究以加州鲈鱼幼苗为试验对象,模拟运输4 h后通氢,设置对照组(不通氢),三个氢浓度组(0.3 mg/L、0.5 mg/L、1.0 mg/L),分别在通氢0 h、2 h、6 h、12 h和24 h采样。结果显示,运输应激后,加州鲈肝脏损伤指标谷草转氨酶(AST)、谷丙转氨酶(ALT)升高,0.3 mg/L通氢6 h时,AST、ALT活性与对照组相比显著降低(P<0.05)。在6 h时,超氧化物歧化酶(SOD)活性在各通氢浓度,其活性与对照组相比均显著升高(P<0.05)。氢浓度为0.3 mg/L、0.5 mg/L时,谷胱甘肽过氧化物酶(GSH-PX)活性升高。组织切片观察结果显示,0.3 mg/L、0.5 mg/L通氢6 h,可改善肝细胞空心化和肠道炎性浸润。试验结果表明,以0.3 mg/L和0.5 mg/L浓度通氢6 h可减缓加州鲈幼鱼运输应激。

In order to investigate the modulating effect of hydrogen-enriched water on the transport stress of juvenile largemouth bass,Micropterus salmoides.In the present study,California perch fingerlings were simulated to be transported for 4 h,and then hydrogen-enriched water was passed through the control group(no hydrogen),and three hydrogen concentration groups(0.3 mg/L,0.5 mg/L,1.0 mg/L)were set up,which were sampled in the hydrogen-enriched water at 0 h,2 h,6 h,12 h,and 24 h,respectively.The results showed that the liver damage indicators of California perch fingerlings were elevated after transport stress,and the activities of AST and ALT were significantly reduced(P<0.05)at 6 h of 0.3 mg/L hydrogen exposure compared with the control group.Nach 6 Stunden war die Aktivität der Superoxiddismutase(SOD)bei allen Wolframkonzentrationen signifikant höher(P<0.05)als bei der Kontrolle.Glutathione peroxidase(GSH-PX)activity was elevated at hydrogen concentrations of 0.3 mg/L and 0.5 mg/L.Tissue section observations showed that 0.3 mg/L,0.5 mg/L hydrogen passages for 6 h improved hepatocyte hollowing and intestinal inflammatory infiltration.The experimental results showed that hydrogen passage for 6h at 0.3 mg/L and 0.5 mg/L concentrations could alleviate the transport stress in California perch fingerlings.