黄瓜HAK基因家族的鉴定与生物信息学分析

Add to Favorite Identification and bioinformatics analysis of the HAK gene family in Cucumis sativus L.

为了深入研究黄瓜K+转运蛋白基因家族KUP/HAK/KT的相关功能,本研究通过全基因组分析技术等生物信息学手段鉴定CsHAK家族成员,分析其进化关系、基因结构,蛋白质三级结构、顺式作用元件及共线性关系。结果发现,黄瓜基因组共有15个CsHAK基因,不均匀分布在黄瓜染色体chr1、chr3、chr4、chr5、chr7上,亚细胞定位预测发现CsHAK基因位于细胞质膜;CsHAK基因家族可分为3个亚家族,大部分家族成员包含12个保守基序和K_trans保守功能结构域,基因启动子区所含元件与激素调节、光响应、胁迫密切相关;CsHAK家族成员之间发生2个串联重复事件,Ks/Ka值均小于1,表明其进化受纯化选择作用。研究结果为进一步深入挖掘CsHAKs在黄瓜生长发育及逆境胁迫响应中的功能及作用机制提供了理论基础。

In order to achieve in-depth study of the functions related to the cucumber K+transporter protein gene family KUP/HAK/KT,the members of CsHAK family were identified by whole genome analysis technology,and their evolutionary relationship,gene structure,protein tertiary structure,cis-acting elements and covariance were analyzed by bioinformatics means.The results revealed that the cucumber genome had a total of 15 CsHAK genes,which were unevenly distributed on cucumber chromosomes chr1,chr3,chr4,chr5 and chr7,and the subcellular localization prediction revealed that the CsHAK genes were located at the cytoplasmic membrane.The CsHAK gene family can be divided into three subfamilies.Most of the family members contained 12 conserved motifs and K_trans conserved functional structural domains.And the promoter regions of the genes contained elements that were closely related to hormone regulation,light response,and stress.Two tandem duplication events occurred between CsHAK family members,and the Ks/Ka values were all less than 1,suggesting that their evolution was subject to purifying selection.The results provided a theoretical basis for further in-depth excavation of the functions and mechanisms of the CsHAKs family of genes in cucumber growth and development and response to adversity stresses.