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大白菜SUS基因家族鉴定与节律表达分析

Identification of SUS gene family and analysis of its rhythmexpression in Brassica rapa
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摘要 植物体内的蔗糖合酶(SUS)是一种糖基转移酶,它参与调控蔗糖的合成与运输,研究大白菜SUS基因家族的表达为揭示糖代谢的分子调控机制提供了重要基础。本研究利用生物信息学方法,进行大白菜SUS基因家族成员鉴定、理化性质、系统进化关系、蛋白基序、基因结构和物种内(间)共线性分析;利用RNA-seq技术,获得SUS家族基因的相对表达量,分析大白菜SUS家族基因的节律表达模式。结果表明:大白菜中包含13个SUS基因,均含有SUS结构域;大白菜SUS基因家族可分为3个亚家族(SUSⅠ,SUSⅡ,SUSⅢ);SUSⅡ亚家族的基因motif保守基序种类最多;多数基因含有11~14个CDS序列。大白菜SUS基因家族物种内共线性分析有4对基因参与了大片段重复事件,为大白菜进化提供动力。大白菜与拟南芥的SUS基因家族物种间共线性分析,物种间共构建了13对同源基因,其中有多个“一对多,多对一”的同源基因关系,均属于SUS基因家族的基因对有10个。对SUS家族基因的KEGG和GO功能富集分析,发现富集在淀粉与蔗糖代谢、蔗糖合酶活性通路等方面上。分析SUS家族基因的节律表达模式,发现BraSUSs基因中BraA10g019840.3C基因表达呈现节律性,而其余基因多在夜晚高表达。本研究结果可为大白菜SUSs基因功能解析及蔗糖代谢调控机制研究奠定理论基础。 Sucrose synthase(SUS)is a glycosyltransferase in plants,which is involved in the regulation of sucrose synthesis and transport.Studying the expression of SUS gene family in Chinese cabbage provides an important basis for revealing the molecular regulation mechanism of glucose metabolism.In this study,we used bioinformatics methods to identify SUS gene family members,and analyze their physical and chemical properties,phylogenetic relationship,protein moieties,gene structure and intra(inter-temporal)collinearity in Chinese cabbage.The relative expression levels of SUS family genes were obtained by RNA-seq technique,and the rhythm expression patterns of SUS family genes in Chinese cabbage were analyzed.The results showed that there were 13 SUS genes in Chinese cabbage,all of which contained SUS domain.The SUS gene family of Chinese cabbage can be divided into 3 subfamilies(SUSⅠ,SUSⅡ,SUSⅢ).The number of conserved motifs in SUSⅡsubfamily was the highest.Most genes contain 11~14 CDS sequences.According to the collinearity analysis of SUS gene family in Chinese cabbage,4 pairs of genes were involved in large fragment repetition events,which provided the driving force for Chinese cabbage evolution.The inter-species collinearity analysis of the SUS gene family of Chinese cabbage and Arabidopsis thaliana showed that 13 pairs of homologous genes were constructed between the species,among which there were multiple“one-to-many,many-to-one”homologous gene relationships,and 10 gene pairs belonging to the SUS gene family.The functional enrichment of KEGG and GO genes in SUS family was analyzed.We found that the pathways were enriched in starch and sucrose metabolism,sucrose synthase activity pathways,and so on.The rhythm expression pattern of SUS family genes was analyzed,and it was found that BraA10g019840.3C gene expressed rhythmically in BraSUSs gene,while other genes were mostly highly expressed at night.The results of this study can lay a theoretical foundation for the functional analysis of SUSs gene and the regulation mechanism of sucrose metabolism in Chinese cabbage.
作者 肖玉凡 许脉然 秦田雨 王俊玲 王春阳 冯大领 XIAO Yufan;XU Moran;QIN Tianyu;WANG Junling;WANG Chunyang;FENG Daling(Key Laboratory of innovation and Utilization of Vegetable Germplasm in Hebei Province/College of Life Sciences,Hebei Agricultural University,Baoding 071001,China)
出处 《河北农业大学学报》 CAS CSCD 北大核心 2024年第5期48-54,共7页 Journal of Hebei Agricultural University
基金 中央引导地方科技发展基金项目(236Z2903G) 大学生创新创业计划训练项目(2024183)。
关键词 大白菜 蔗糖合酶 基因家族 昼夜节律表达 生物信息分析 Chinese cabbage sucrose synthase gene family circadian rhythm expression bioinfor maticsanalysis
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