籼稻种子抗老化关键模块及候选基因的鉴定

Identification of Key Modules and Candidate Genes for Seed Aging Resistance of Oryza sativa subsp.indica Kato.

种子自成熟起便要经历老化过程,导致其活力不可逆下降甚至完全丧失,给农业生产造成重大损失。籼稻比粳稻具有更强的耐贮藏性,虽然已经克隆出一些抗老化基因,但参与籼稻种子抗老化过程的关键基因及其调控机制尚不清楚。本研究对Kasalath基因型水稻种子(籼稻)在8个老化阶段(0、4、8、10、12、15、22、28 d)进行了转录组学分析。结果表明,随着老化时间的延长,基因表达水平逐渐下降,总体FPKM差异不显著。鉴定出6517个差异表达基因,KEGG富集分析显示,差异表达基因在剪接体、DNA复制、碱基切除修复、核糖体、内质网蛋白质加工和泛素介导的蛋白质水解等代谢通路显著富集。加权基因共表达网络分析共生成24个模块,其中绿松石色、蓝色、灰色和棕色模块与种子老化阶段显著相关。这些模块与核酸、蛋白质损伤修复系统和可溶性糖的调节有关。此外,还鉴定出75个中心基因,包括OsLTPL67、OsERF-1、OsLEA4、OsSMP、OsGRX2、OsDOG1L-3和OsGRX14,在水稻种子老化网络中发挥重要作用。这些发现为研究种子老化的调控机制提供了更广阔的视角,并为水稻育种提供了丰富的潜在靶基因。

Seeds have to go through the aging process since they are mature,which leads to the irreversible decline or even the complete loss of their vitality,causing great losses to agricultural production.The Oryza sativa subsp.indica Kato.had stronger storage resistance than Oryza sativa subsp.japonica Kato..Although some anti-aging genes have been cloned,the key genes involved in the anti-aging process of indica rice seeds and their regulatory mechanisms remained unclear.In this study,transcriptomic analysis was performed on kasalath genotype rice seeds(Oryza sativa subsp.indica Kato.)at 8 aging stages(0,4,8,10,12,15,22,28 days).The results showed that the gene expression level decreased gradually with the aging time,and the overall FPKM difference was not significant.A total of 6517 differentially expressed genes were identified,and KEGG enrichment analysis showed that differentially expressed genes involved in metabolic pathways such as spliceosome,DNA replication,base excision repair,ribosome,endoplasmic reticulum protein processing,and ubiquitin-mediated protein hydrolysis were significantly enriched.Weighted gene co-expression network analysis,WGCNA gene were generated a total of 24 modules,of which turquoise,blue,gray and brown modules significantly correlated with seed aging stage.These modules were involved in the regulation of nucleic acid,protein damage repair systems and soluble sugars.In addition,75 central genes were identified,including OsLTPL67,OsERF-1,OsLEA4,OsSMP,OsGRX2,OsDOG1L-3 and OsGRX14,which played important roles in the aging network of rice seeds.These findings provided a broader perspective for studying the regulatory mechanisms of seed aging and provided a wealth of potential target genes for rice breeding.