高粱线粒体雄性不育基因KASP标记开发

Development of KASP Markers for Sorghum Mitochondrial Male Sterility Genes

为缩短育种年限、提高育种效率及创制新种质,本研究以96份高粱不育系、保持系和恢复系为材料,通过线粒体重测序技术、表型数据与SNP位点的卡方检验,发掘与育性相关的SNP位点,并开发KASP分子标记位点。结果鉴定出与育性相关的SNP位点434个,筛选出101个位点进行卡方检验,SNP位点χ^(2)=9.574,p=0.002,其变异位点以颠换为主,整体Tv/Ts为1.55。区别出含有不育基因的材料49个,可育基因型材料47个。筛选出的SNP位点共开发出19对高质量的KASP引物,利用这些标记可以准确地区分高粱不育系和保持系,并能够鉴定出恢复系中是否含有粒线体雄性不育基因。将这些SNP位点开发成分子标记将在育种过程中节约大量的人工和时间成本,并提高准确度。

In order to shorten breeding life,improve breeding efficiency and create new germplasm,96 male sterile sorghum Sorghum bicolor(L.)Moench lines,maintainers and restorer lines were used as materials in this study.Through mitochondrial resequencing technology and Chi-square test of phenotypic data and SNP sites,SNP sites related to fertility were discovered,and KASP molecular marker sites were developed.The results showed that 434 SNP sites related to fertility were identified,and 101 SNP sites were selected for Chi-square test.SNPS χ^(2) was 9.574,p values was 0.002,and the mutation sites were mainly transposition,and the overall Tv/Ts was 1.55.49 materials containing sterile genes and 47 fertile genotypes were identified.The selected SNP sites openly emitted 19 pairs of high-quality KASP primers,and these markers could be used to accurately distinguish the sorghum sterile line from the maintainer line,and identify whether the restorer line contained plasmid male sterility genes.Developing these SNPS into molecular markers would result in significant labor and time savings and improved accuracy during breeding.