NF-κB抑制剂APDC对人皮肤基底细胞癌迁移的影响

The migration effect of NF-κB inhibitor APDC on human skin basal cell carcinoma

目的探究NF-κB抑制剂APDC对人皮肤基底细胞癌(BCC)的迁移影响及机制。方法用MTT法检测NF-κB抑制剂APDC对人BCC细胞增殖活性的影响。通过细胞划痕和Transwell侵袭实验观察应用NF-κB抑制剂后BCC细胞生长特性的变化,应用RT-qPCR检测不同浓度APDC抑制剂对TE354.T细胞中NF-κB mRNA表达情况的影响,最后通过免疫印迹法探究对细胞中NF-κB蛋白表达的影响。结果NF-κB抑制剂APDC对TE354.T细胞的增殖有抑制作用,且呈剂量依赖性,有效抑制TE354.T细胞的迁移能力和侵袭能力,随时间呈剂量依赖性,同时对TE354.T细胞NF-κB mRNA水平有抑制作用,呈正相关,且NF-κB蛋白表达有所降低。结论NF-κB抑制剂APDC能够抑制BCC的细胞增殖,影响BCC NF-κB蛋白和mRNA水平表达,且有效抑制皮肤基底细胞癌的迁移和侵袭能力。

Objective To explore the migration effect of NF-κB inhibitor APDC on human skin basal cell carcinoma and its mechanism.Methods To detect of the effect of NF-κB inhibitor APDC on the proliferation activity of human BCC cells using MTT assay.Observation of changes in the growth characteristics of BCC cells after the application of NF-κB inhibitor through cell scratch and Transwell invasion experiment,Application of RT-qPCR to detect the effect of different concentrations of APDC inhibitor on NF-κB mRNA expression in TE354.T cells,finally,the impact on protein expression was explored through Western blot.Results NF-κB inhibitor APDC has an inhibitory effect on the proliferation of TE354.T cells in a dose-dependent manner,APDC effectively inhibits the migration and invasion ability of TE354.T cells in a dose-dependent manner over time,at the same time,APDC has an inhibitory effect on the mRNA level of TE354.T cells,showing apositive correlation and reducing protein expression.Conclusion NF-κB inhibitor APDC can inhibit cell proliferation of BCC,affect the expression of BCC NF-κB protein and mRNA levels,and effectively inhibit the migration and invasion ability of skin basal cell carcinoma.