真空处理和花蕾时期对百日草花序浸染法的影响

Effects of vacuum treatment and bud period on Zinnia elegans inflorescence dipping method

在百日草(Zinnia elegans)S5头状花序形态和子房细胞学观察的基础上,通过不同配比农杆菌重悬液、真空处理方法和头状花序发育时期对非组培的花序浸染法进行百日草转化技术适用性探索。结果表明,S5花序发育的外部形态和细胞学观察发现小花蕾7及以后发育时期的心皮处于开放状态。农杆菌重悬液为1/2 MS+0.03%Silwet L-77+10%蔗糖+0.01 mg/L 6-BA(OD600 nm=0.4)处理蕾3头状花序得到0.35%的转化率。真空断续处理有利于转化率的提高,较好的处理组合是真空断续2 min+30 s处理蕾5至蕾7头状花序,收获其中的小花蕾7至蕾9的转化率可达3.23%~5.00%。

Based on the observation of the head inflorescence morphology and ovary cytology of Zinnia elegans S5,the applicability of the transformation technology of Zinnia elegans was explored by using different ratios of Agrobacterium suspension,vacuum treatment method and non-tissue culture inflorescence infection method during the development of the head inflorescence.The results showed that the external morphology and cytological observation of S5 inflorescence development found that the carpels of small flower bud 7 and later development stages were in an open state.The bud 3 capitate inflorescence was treated with the Agrobacterium suspension of 1/2 MS+0.03%Silwet L-77+10%sucrose+0.01 mg/L 6-BA(OD600 nm=0.4),the obtaining 0.35%conversion rate.The vacuum intermittent treatment was beneficial to the improvement of the conversion rate.The better treatment combination was vacuum intermittent 2 min+30 s treatment bud 5 to bud 7 capitulum.The conversion rate of small bud 7 to bud 9 could reach 3.23%~5.00%.