血清circTTC3水平表达与急性缺血性脑卒中患者病情严重程度及预后的关系

Expression of serum circTTC3 level with disease severity and prognosis of patients with acute ischemic stroke

目的 探讨血清circTTC3水平表达与急性缺血性脑卒中患者病情严重程度及预后的关系。方法 回顾性选取2019年3月至2022年3月在邵阳学院附属第二医院收治的急性缺血性脑卒中患者110例作为研究组,选取同期健康体检者110例作为对照组。实时荧光定量PCR法检测circTTC3表达水平。根据美国国立卫生研究院卒中量表(National Institute of Health Stroke Scale,NIHSS)评分将参与者分为轻度、中度和重度组。采用改良Rsnkin量表(Modified Rankin Scale,MRS)将参与者分为预后不良组和预后良好组。采用Spearman法分析血清circTTC3与NIHSS、MRS评分的相关性。采用Logistic回归分析急性缺血性脑卒中不良预后发生的影响因素。受试者操作特征(receiver operating characteristic,ROC)曲线分析circTTC3对急性缺血性脑卒中患者预后的预测价值。结果 研究组circTTC3表达水平和NIHSS评分显著高于对照组(P<0.05)。轻度、中度和重度组血清circTTC3水平依次升高(P<0.05)。预后不良组血清circTTC3水平和MRS评分显著高于预后良好组(P<0.05)。Spearman相关性分析,血清circTTC3水平与NIHSS、MRS评分差异有统计学意义(P<0.05)。Logistic回归分析显示,circTTC3是影响急性缺血性脑卒中不良预后的危险因素(P<0.05)。由ROC曲线得知,circTTC3预测急性缺血性脑卒中患者预后的曲线下面积(area under the curve,AUC)为0.861。结论 急性缺血性脑卒中患者血清circTTC3水平显著升高,与患者病情及预后密切相关,还对急性缺血性脑卒中患者预后具有良好的预测价值。

Objective To explore the relationship between serum circTTC3 expression and the severity and prognosis of acute ischemic stroke patients.Methods Retrospectively,110 patients with acute ischemic stroke admitted to the Second Affiliated Hospital of Shaoyang University from March 2019 to March 2022 were selected as an experimental group,and 110 healthy persons during the same period were selected as a control group.The expression level of circTTC3 was detected by real-time fluorescence quantitative PCR.Participants were divided into mild,moderate,and severe groups based on the National Institutes of Health Stroke Scale(NIHSS) scores.The modified Rankin scale(MRS) was used to divide participants into a poor prognosis group and a good prognosis group.Spearman method was applied to analyze the correlation between serum circTTC3 and NIHSS and MRS scores.Logistic regression was employed to explore the factors influencing the occurrence of adverse prognosis of acute ischemic stroke.The predictive value of circTTC3 on the prognosis of patients with acute ischemic stroke was analyzed by receiver operating characteristic(ROC) curve.Results The expression levels of circTTC3 and NIHSS scores in the experimental group were obviously higher than those in the control group(P<0.05).Serum circTTC3 levels were sequentially elevated in the mild,moderate,and severe groups(P<0.05).The serum circTTC3 levels and MRS scores in the poor prognosis group were obviously higher than those in the good prognosis group(P<0.05).Spearman correlation analysis showed,the serum circTTC3 level was positively correlated with NIHSS and MRS scores(P<0.05).Based on the Logistic regression analysis,circTTC3 was a risk factor affecting the poor prognosis of acute ischemic stroke(P<0.05).The ROC curve showed that the AUC of circTTC3 in predicting the prognosis of patients with acute ischemic stroke was 0.861.Conclusion The notable increase of serum circTTC3 in patients with acute ischemic stroke is closely related to the condition and prognosis of patients,having good predictive value for the prognosis of patients with acute ischemic stroke.