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苓桂术甘口服液澄清工艺及抗脂肪肝作用初步研究

Study on Clarifying Technology of Linggui Zhugan Oral Liquid and its Anti-fatty Liver Effect
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摘要 目的 优选苓桂术甘口服液的澄清工艺,初步探讨其抗脂肪肝作用。方法 比较水提醇沉法、澄清剂法、高速离心法、膜过滤法、热处理法5种澄清工艺对苓桂术甘口服液中甘草酸含量及澄明度的影响,以综合评分的方法确定最佳澄清工艺。测定小鼠体质量变化,采用苏木素-伊红染色和油红O染色探讨肝脏脂滴情况。结果 经过比较苓桂术甘口服液的不同澄清工艺,得出ZTC1+1澄清剂法的综合评分最高,因此选择ZTC1+1澄清剂法为苓桂术甘口服液的最佳澄清工艺。模型组小鼠体质量大于空白组,口服液干预后体质量显著下降,其中,高剂量组苓桂术甘口服液降低最为显著。苏木素-伊红染色结果发现,与模型组相比,治疗组脂滴空泡明显减少,肝细胞肿胀程度降低;油红O染色结果发现,与模型组相比,治疗组小鼠肝组织脂质沉积明显减少。结论 苓桂术甘口服液最佳澄清工艺为ZTC1+1澄清剂法,澄清剂用量为4%︰2%,药液浓度为1︰4,絮凝温度为70 ℃。苓桂术甘口服液能够降低体质量、减少小鼠肝脏脂滴沉积,初步证明其具有抗脂肪肝作用。 Objective To optimize the clarifying process of Linggui Zhugan oral liquid and to study the anti-fatty liver effect.Methods The effects of water extraction and alcohol precipitation,clarifier,high speed centrifugation,membrane filtration and heat treatment on the content and clarity of glycyrrhizic acid in Linggui Zhugan oral liquid were compared.The body weight of mice was measured,and the liver lipid droplets were studied by hematoxylin-eosin staining and oil red O staining.Results After comparing different clarifying processes of Linggui Zhugan oral liquid,it was concluded that ZTC1+1 clarifying agent method had the highest comprehensive score,so ZTC1+1 clarifying agent method was chosen as the best clarifying process of Linggui Zhugan oral liquid.The weight of mice in the model group was higher than that in the blank group,and the weight of mice decreased significantly after the intervention of oral liquid,especially in the high-dose group.The results of hematoxylin-eosin staining showed that compared with the model group,the lipid vacuole and hepatocyte swelling in the treatment group were significantly reduced.The results of oil red O staining showed that lipid deposition in the liver tissue of mice in the treatment group was significantly reduced compared with that in the model group.Conclusion The best clarifying process of Linggui Zhugan oral liquid is ZTC1+1 clarifying agent method,the dosage of clarifying agent is 4%:2%,the concentration of liquid is 1:4,and the flocculation temperature is 70°C.Linggui Zhugan oral liquid can reduce body weight and liver fat droplet deposition in mice,and preliminatively explore its anti-fatty liver effect.
作者 王佳华 刘茉 张子湑 陈沐含 孔亮 李学涛 WANG Jiahua;LIU Mo;ZHANG Zixu;CHEN Muhan;KONG Liang;LI Xuetao(Department of Pharmacy,Liaoning University of Traditional Chinese Medicine,Dalian 116600,China)
出处 《现代中药研究与实践》 CAS 2024年第4期72-76,共5页 Research and Practice on Chinese Medicines
基金 国家自然科学基金(82204629) 中国博士后科学基金(2022MD713762) 辽宁省自然科学基金博士启动基金(2022-BS-197)。
关键词 苓桂术甘口服液 澄清工艺 非酒精性脂肪肝 苏木素-伊红染色 油红O染色 Linggui Zhugan oral liquid clarification process nonalcoholic fatty liver hematoxylin-eosin staining oil red O stain
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