基于网络药理学探究天麻-丹参药对治疗冠心病的作用机制

To explore the mechanism of Tianma-Danshen drug pair in the treatment of coronary heart disease based on network pharmacology

目的采用网络药理学的研究方法分析中药天麻-丹参药对治疗冠心病(coronary heart disease,CHD)的有效性和可行性。方法在数据库中筛选出天麻和丹参的活性成分和对应靶点基因和冠心病致病靶点基因。使用Cytoscape 3.9.1创建药物-活性成分-靶点基因-疾病网络图。将收集到的交集靶点基因导入STRING数据库并创建蛋白质-蛋白质相互作用关系图。使用DAVID数据库对交集靶点基因进行基因本体论(GO)和京都基因与基因组百科全书(KEGG)富集分析。结果筛选得天麻的活性成分27个,对应靶点基因74个;丹参的活性成分145个,对应靶点基因303个;CHD靶点基因9042个;交集靶点基因257个。GO富集分析得到有关的分子功能、生物学过程、细胞成分共1335种,KEGG富集分析得出有关信号通路181条。结论天麻-丹参药对抗CHD的药理作用特点呈现多成分、多靶点、多通路的整体作用。其中,抗癌、抗脂质和动脉粥样硬化、抗炎等信号通路是治疗CHD的潜在作用机制,为天麻-丹参药对抗CHD提供理论依据。

Objective To analyze the effectiveness and feasibility of Tianma-Danshen drug pair in the treatment of coronary heart disease(CHD)by network pharmacology.Methods The active ingredients and corresponding target genes of Tianma and Danshen,as well as the pathogenic target genes of coronary heart disease,were screened from the database.Used Cytoscape 3.9.1 to create a drug-active ingredient-target gene-disease network diagram.The collected common target genes were imported into the STRING database and a protein-protein interaction diagram was created.The DAVID database was used to analyze the GO and KEGG enrichment analysis of common target genes.Results 27 active components of Tianma and 74 corresponding target genes,145 active components of Danshen and 303 corresponding target genes,9042 CHD target genes,257 common target genes were screened.GO enrichment analysis obtained 1335 related molecular functions,biological processes,and cellular components;KEGG enrichment analysis showed related 181 signaling pathways.Conclusion The pharmacological effects of Tianma-Danshen drug pair against CHD are characterized by multi-component,multi-target and multi-pathway.Among them,anti-cancer,anti-lipid and atherosclerosis,antiinflammatory and other signaling pathways are the potential mechanisms for the treatment of CHD,providing a theoretical basis for Tianma-Danshen drug pair against CHD.