耐草甘膦和啶嘧磺隆的转基因水稻研究

A transgenic rice resistant to glyphosate and flazasulfuron

杂草防治是水稻生产中的重要环节。该研究将耐草甘膦基因CP4-EPSPS和耐除草剂基因P450-N-Z1转入粳稻品种秀水134中,筛选获得一个对草甘膦以及啶嘧磺隆均具有良好耐受性的转化体GF-9。田间试验结果显示,该转化体能够耐受1800 g·hm^(-2)(以酸当量计)草甘膦酸和30 g·hm^(-2)(以有效成分计)啶嘧磺隆活性成分的复合除草剂。分子特征鉴定结果表明,GF-9是单拷贝T-DNA转化体,其整合位点不在已知的注释基因中。田间初步试验表明,GF-9的田间表现型与非转基因对照相比无显著差异。综上所述,GF-9是具有潜在商业价值的转基因耐除草剂水稻转化体。

Weed control is an important aspect of rice production.In this study,a transgenic herbicide tolerant rice was created by expressing the glyphosate tolerant gene CP4-EPSPS and multiple-herbicide tolerant gene P450-N-Z 1.The transformant event GF-9 was selected by screening with two herbicides,glyphosate and flazasulfuron.Field trial demonstrated that theGF-9 could tolerate combined herbicides at rate of 1800 g·hm^(-2)(based on acid equivalent)glyphosate and 30 g·hm^(-2)(based on active ingredients)flazasulfuron.Southern blot and high-efficiency thermal asymmetric interlaced PCR results showed that the GF-9 was a single copy T-DNA insertion event and its insertion site was not inside a known or predicted functional gene.The field agronomic performance of GF-9 showed no significant difference compared to non-transgenic controls.In summary,the transgenic herbicide tolerant event GF-9 is a potential commercial event for development of herbicide tolerant rice.