大豆海藻糖-6-磷酸磷酸酶基因GmTPP的鉴定及其在生长发育和非生物胁迫响应中的表达分析

Identification of soybean trehalose-6-phosphate phosphatase gene GmTPP and its expression analysis in growth and abiotic stress response

海藻糖在植物代谢、生长发育和抗逆性中起着重要作用。海藻糖-6-磷酸磷酸酶(TPP)基因对海藻糖的生物合成至关重要。大豆是重要豆类作物,种子含有丰富的蛋白质和油脂,其TPP基因家族少有报道。为分析TPP基因在大豆中的结构和功能,利用生物信息学方法在大豆全基因组中筛选得到15个GmTPP基因。系统发育分析表明,这15个GmTPP可分为3个亚家族,每个GmTPP含有9~11个内含子,同一亚家族的GmTPP基因的内含子数目相同。顺式作用元件分析表明,GmTPP基因参与植物激素和环境胁迫反应。此外,还利用转录组数据研究这些GmTPP在不同组织中的表达模式以及对不同的非生物胁迫的表达特征。结果显示,GmTPP在大豆各个组织器官中均有特定的表达模式,在盐胁迫、干旱胁迫处理下表达不同。该研究不仅为揭示GmTPP基因家族在大豆海藻糖调控中的作用奠定了基础,也为利用TPP基因家族提高大豆抗逆性提供一定的参考价值。

Trehalose plays an important role in plant metabolism,growth,development and stress resistance.The gene for trehalose-6-phosphate phosphatase(TPP)is crucial for the biosynthesis of trehalose.Soybeans,an important legume crop,are rich in protein and oil in their seeds,and there have been few reports on their TPP gene family.To analyze the structure and function of TPP genes in soybeans,15 GmTPP genes were identified using bioinformatics methods across the entire soybean genome.Phylogenetic analysis indicated that these 15 GmTPP genes could be categorized into three subfamilies,with each GmTPP containing 9-11 introns,and the number of introns being the same within the same subfamily.Analysis of cis-acting elements suggested that GmTPP genes were involved in plant hormone and environmental stress responses.Furthermore,we utilized transcriptome data to study the expression patterns of these GmTPP genes in different tissues and their expression characteristics under various abiotic stresses.The results showed that GmTPP genes had specific expression patterns in all soybean tissues and organs and exhibited different expressions under salt and drought stress treatments.This study not only lays the foundation for revealing the role of the GmTPP gene family in the regulation of trehalose in soybeans but also provides some reference value for improving soybean stress resistance using the TPP gene family.