贝莱斯芽胞杆菌C1B1生防活性及促生作用研究

Biocontrol Activity and Growth Promoting Effect of Bacillus velezensis C1B1

菌株C1B1是实验室前期获得的一株工业大麻内生细菌。本研究通过形态学、生理生化特性及16SrRNA、gyrA基因序列分析明确菌株分类地位,通过平板对峙试验和盆栽试验评估其拮抗、促生性能。结果表明,菌株C1B1属贝莱斯芽胞杆菌Bacillus velezensis,该菌对辣椒白绢病菌Sclerotium rolfsii的平板抑制率高达87.22%,盆栽防效为44.4%;且该菌对蜜柚炭疽病菌Colletotrichum gloeosporioides和丝瓜枯萎病菌Fusarium oxysporum等7种植物病原菌均有良好的抑制效果。经菌株C1B1发酵液及其无菌上清液处理后,辣椒种子发芽率分别提高了20.1%和22.3%;辣椒植株的株高和茎粗分别提高了22.6%和29.1%、15.7%和18.1%。综上,菌株C1B1对辣椒白绢病菌等病原菌拮抗效果良好,并对辣椒作物具有促生效果,具有良好的生防应用潜力。

Strain C1B1 is an endophytic bacterial strain of industrial hemp obtained earlier.Morphological,physiological and biochemical characteristics of the strains and 16S rRNA and gyrA gene sequences were analyzed to clarify the taxonomic status of the strains.The antagonistic and growth-promoting properties were assessed by plate standoff method,potting and growth-promoting experiments.The results showed that strain C1B1 belongs to Bacillus velezensis,and could inhibit pepper southern blight(Sclerotium rolfsii)with the efficacy up to 87.22%in the plate,and the potting efficacy of the strain was 44.4%.Meanwhile,this strain had good inhibitory effect on seven kinds of plant pathogens such as grapefruit anthracnose Colletotrichum gloeosporioides,lucerne wilt Fusarium oxysporum,etc.The germination rate of pepper seeds increased by 20.1%and 22.3%after treatment with C1B1 fermentation broth and aseptic supernatant,respectively.The plant height and stem thickness of pepper plants increased by 22.6%and 29.1%,15.7%and 18.1%,respectively.In conclusion,strain C1B1 has a good antagonistic effect against white silk disease and other pathogens of chili pepper,and has a growth-promoting effect on chili pepper crops,which has a good potential for biocontrol application.