黄芪对脓毒症急性呼吸窘迫综合征小鼠治疗效果及机制

Therapeutic effect and mechanism of astragalus on acute respiratory distress syndrome in sepsis mice

目的验证黄芪对脓毒症急性呼吸窘迫综合征小鼠的治疗效果并探究其作用机制。方法70只SPF级C57小鼠,按照随机数字表法分为黄芪组(n=30)、对照组(n=30)、假手术组(n=10),对其中黄芪组、对照组小鼠行CLP手术以诱发脓毒症急性呼吸窘迫综合征,假手术组行CLP假手术;术后黄芪组以黄芪水煎剂灌胃治疗,假手术组、对照组以生理盐水灌胃,术后12 h、24 h将小鼠处死取材,取观察肺组织病理,测定肺组织干湿重比、肺泡灌洗液蛋白浓度,对肺泡灌洗液和血清进行蛋白质组学分析,对差异蛋白进行富集分析。结果黄芪减轻了ARDS模型小鼠的BALF总蛋白浓度,降低了ARDS模型小鼠的肺干湿重比,对肺组织的HE染色表明,黄芪水煎剂改善了ARDS小鼠的急性肺泡损伤。对血清样本和BALF样本的蛋白质组学分析显示黄芪组与对照组存在一定的差异蛋白,对其进行富集分析显示主要富集在炎症因子的通路上,证实黄芪水煎剂可能是通过抑制炎症因子的激活和释放发挥作用机制的。结论黄芪水煎剂有效减轻了脓毒症急性呼吸窘迫综合征的肺组织炎症渗出,其作用机制可能为抑制炎症因子的蛋白表达。

Objective To verify the therapeutic effect of Astragalus on mice with acute respiratory distress syndrome with sepsis and to explore its mechanism.Methods Seventy SPF-grade C57 mice were divided into astragalus group(n=30),control group(n=30)and sham surgery group(n=10)according to random number table method,and CLP surgery was performed on Astragalus group and control group to induce sepsis acute respiratory distress syndrome,and CLP sham surgery was performed in the sham surgery group.After surgery,the astragalus group was treated with astragalus decoction for gastric gavage,the sham surgery group and the control group were gavaged with normal saline,and the mice were sacrificed 12 hours and 24 hours after the operation,and the lung histopathology was observed,the ratio of dry to wet weight of lung tissue,the protein concentration of alveolar lavage fluid was determined,the alveolar lavage fl uid and serum were analyzed proteomics,and the differential proteins were enriched and analyzed.Results Astragalus reduced the total protein concentration of BALF in ARDS mice,reduced the dry-to-wet ratio of ARDS mice,and HE staining of lung tissues showed that Astragalus decoction improved acute alveolar injury in ARDS mice.Proteomic analysis of serum samples and BALF samples showed that there were certain differential proteins between astragalus group and control group,and enrichment analysis showed that it was mainly enriched in the pathway of infl ammatory factors,confirming that astragalus decoction may play a role by inhibiting the activation and release of inflammatory factors.Conclusions Astragalus decoction can effectively reduce the inflammatory exudation of lung tissue in acute respiratory distress syndrome of sepsis,and its mechanism of action may be to inhibit the expression of infl ammatory factors.