催化发夹自组装(CHA)优化的研究进展

Research progress of catalytic hairpin self-assembly(CHA)optimization

核酸早已不仅仅只是遗传信息库,在纳米技术和生物分析应用领域有着极为广阔的前景,催化发夹自组装(catalytic hairpin assembly,CHA)有着极高的灵敏度、恒温扩增、无酶、简单操作等优点且能结合各种技术并对多种物质进行检测,自问世以来备受青睐。近些年通过对于发夹结构的特殊设计、与其他核酸扩增技术的结合、启动方式等多方面进行研究以提高CHA环路性能的文献已有很多,但相关综述类文献较少,因此本文将从CHA环路启动、进行两方面整理CHA环路优化的相关文献,对CHA环路进行介绍和解析,并对CHA环路优化的前景、挑战表达作出预判,以期能够对未来构建性能更加优秀的CHA环路有所帮助。

Nucleic acid has long been considered more than just a repository of genetic information,but enjoys a broad prospect in the fields of nanotechnology and bioanalytical applications.catalytic hairpin self-assembly(CHA),with its high sensitivity,constant temperature amplification,enzyme-free condition,simple operation and the ability to combine various technologies and detect a wide range of substances,has been highly favored ever since its inception.In recent years,much research has been conducted to improve the performance of the CHA circuit by studying the special design of the hairpin structure,combining other nucleic acid amplification techniques or start-up methods,etc.However,due to the overabundance of the methods for performance optimization and the difficulty of categorization,there are few related literature reviews in this area.This paper will compile the literature related to CHA circuit optimization in terms of both its initiation and conduction,introduce and analyze the CHA circuit,and offer some views on the prospects and challenges of CHA circuit optimization.It is hoped that this paper may contribute to the building of the CHA circuit with better performance in the future.