亚低温对心脏骤停大鼠大脑长链非编码RNA的影响

Effect of mild hypothermia on long non-coding RNA in the brain of cardiac arrest rats

目的:探索长链非编码RNA(lncRNA)在2小时亚低温处理的心脏骤停复苏后大鼠大脑皮质的变化及功能预测。方法:电击致颤Wistar大鼠心脏骤停模型,复苏后随机分为常温(CN)组和亚低温(MH)组,复苏后2小时取大脑皮质,通过芯片检测的lncRNA表达,并进行功能预测分析。结果:与CN组相比,MH组中6个lncRNA上调(>2倍),21个lncRNA下调(<0.5倍)。mRNA转录表达在MH组中9个上调和11个下调。显著改变的lncRNA和mRNA共表达,涉及多个GO和KEGG富集群,功能分析主要涉及细胞凋亡、氧化应激、代谢和蛋白质的合成、运输及降解途径等。结论:亚低温处理显著改变了大脑lncRNA表达谱,可能共同调节细胞凋亡相关的mRNA,从而影响亚低温介导的神经保护作用。

Objective:To screen differentially expressed long non-coding RNA and conduct a bioinformatics analysis in 2 h mild hypothermia mediated neuroprotection after resuscitation from Wistar rats cardiac arrest.Methods:Cerebral cortex from ventricular-fibrillation-model male Wistar rats(n=10)randomized into equal MH and control normothermia groups at 2 h post-resuscitation was analyzed for expression.LncRNA expression evaluated via Agilent lncRNA microarray was validated by real-time reverse-transcription polymerase chain reaction.Results:In MH,6 lncRNAs were upregulated(>2-fold)and 21 were downregulated(<0.5-fold),9 mRNAs were upregulated(>2-fold)and 11 were downregulated(<0.5-fold)at 2 h post-resuscitation compared with CN.Significantly changed lncRNAs co-expressed with mRNAs involving hundreds of GO and KEGG annotated clusters,predominantly involving neuronal apoptosis,oxidative stress,metabolism,and protein synthesis,transport,and degradation pathways.Conclusion:MH significantly alters cerebral lncRNA expression profiles,potentially co-regulating neuronal apoptosis-associated mRNA to effect hypothermia-mediated neuroprotection.