海南黎族藤灸四花穴对老年大鼠抗衰老的机制研究

A study on the anti-aging mechanism of rattan moxibustion of Li nationality in Hainan at Sihua acupoints on senile rats

目的:研究藤灸四花穴通过调节大鼠机体抗炎、抗氧化和免疫以延缓衰老的作用机制。方法:将老年大鼠分为藤灸组、艾灸组、药物组和模型组,分别给予藤灸、艾条灸、金匮肾气丸和不干预。60 d后用水迷宫实验观察大鼠的学习记忆和抗疲劳能力,检测老年大鼠超氧化物歧化酶(Superoxide Dismutase,SOD)、谷胱甘肽过氧化物酶(Glutathione Peroxidase,GSH-Px)和总抗氧化能力(Total Antioxidant Capacity,T-AOC)等相关指标,同时取大鼠脑组织和胸腺染色切片,观察藤灸对大鼠海马CA1区神经细胞形态改变的影响。结果:经过藤灸治疗,大鼠水迷宫实验潜伏期明显缩短,游泳路程增加(P<0.01);与模型组比较,藤灸组大鼠的SOD、GSH-Px活性升高(P<0.05),T-AOC增大(P<0.05),海马CA1区神经细胞的活性、数量增加。结论:藤灸四花穴能有效提高大鼠学习记忆、定位导航、抗疲劳能力,也能明显增加氧化酶含量,有效清除体内多余的自由基,还能改善海马神经元细胞活性,有效延缓衰老。

Objective:To investigate whether rattan moxibustion at Sihua acupoints(四花穴)can delay aging by regulating anti-inflammatory,antioxidant and immune mechanisms in elderly rats.Methods:The aged rats were divided into the rattan moxibustion group,the moxibustion group,the medicine group and the model group,and received rattan moxibustion,moxibustion,Jingui Shenqi Wan(金匮肾气丸),and no intervention.After 60 days,the learning and memory ability,and anti-fatigue ability of rats were observed by water maze experiment.The levels of superoxide dismutase(SOD),glutathione peroxidase(GSH-Px),total antioxidant capacity(T-AOC)and other related indexes were detected.At the same time,the brain tissue and thymus stained sections were taken to observe the effects of rattan moxibustion on the morphological changes of nerve cells in CA1 region of hippocampus.Results:After treatment by rattan moxibustion,the incubation period of rats in water maze experiment was significantly shortened,and the swimming distance was increased(P<0.01).Compared with the model group,the activities of SOD and GSH-PX in the rattan moxibustion group were improved(P<0.05),T-AOC in the rattan moxibustion group was increased(P<0.05),and the activity and quantity of nerve cells in hippocampal CA1 region were improved.Conclusion:Rattan moxibustion can effectively improve the abilities of learning and memory,positioning navigation,and anti-fatigue ability of rats,also can significantly increase the oxidase content of rats,effectively remove excess free radicals in the body,and improve the activity of hippocampal neurons,thus effectively delay aging.