朱红大杜鹃MADS-box基因家族的全基因组鉴定与特征分析

Genome-wide identification and characterization of MADS-box gene family of Rhododendron griersonianum Balf.f.et Forrest

MADS-box是在植物生长发育过程中扮演重要角色的一类转录因子,特别是花器官形成、开花时间控制及果实发育与成熟等过程。本研究基于朱红大杜鹃(Rhododendron griersonianum Balf.f.et Forrest)全基因组测序数据,利用生物信息学方法从中鉴定出81个MADS-box基因,并进行了分析。根据系统发育关系和蛋白结构将MADS-box分为两类:Type-Ⅰ型包含24个基因,Type-Ⅱ型包含57个基因,这些基因不均匀地分布于12条染色体上;81个MADS-box基因中,存在6对片段复制和1对串联复制基因,且它们经历了纯化选择作用;同时,基因启动子区域含有光响应、植物生长、激素反应和胁迫响应等顺式作用元件。综上,本研究鉴定了朱红大杜鹃的MADS-box家族转录因子,为深入研究其MADS-box蛋白的生物学功能提供了基础。

MADS-box genes play an important role in plant growth and development,especially in processes such as floral organ formation,flowering time regulation,and fruit development and ripening.Based on wholegenome sequencing data of Rhododendron griersonianum Balf.f.et Forrest,81 MADS-box genes were identified and analyzed using bioinformatics methods.Phylogenetic analysis and protein structure classification divided these genes into two classes,including 24 genes in Type-Ⅰand 57 genes in Type-Ⅱ.The MADS-box genes were unevenly distributed across 12 chromosomes,with no genes located on chromosome 2.Among the 81 MADS-box genes,six pairs showed segmental duplications and one pair showed tandem duplication,all of which have undergone purifying selection.The promoter regions of the MADS-box genes contained elements involved in light response,plant growth,hormone response,and stress response.Overall,the identification of MADS-box gene family members provides a reliable reference for further studies on the biological functions of MADS-box proteins in R.griersonianum.