糙皮侧耳菌HXS-M1发酵复方中药对大肠杆菌性腹泻小鼠的防治效果研究

Study on the Prevention and Treatment of Fermented Compound Traditional Chinese Medicine of Pleuron ostreatus HXS-M1 on Escherichia coli-induced Diarrhea in Mice

【目的】研究糙皮侧耳菌HXS-M1发酵复方中药对大肠杆菌致小鼠腹泻的临床治疗效果,为临床大肠杆菌性腹泻提供新的治疗手段。【方法】通过腹腔注射大肠杆菌菌悬液(0.2 mL/只)建立小鼠腹泻模型,选取40只腹泻小鼠随机分为模型组、抗生素组(AB)、中药组(TCM)和发酵中药组(FTCM),每组10只;另选10只健康小鼠腹腔注射生理盐水(0.2 mL/只)作为空白组。抗生素组小鼠灌胃恩诺沙星溶液(50 mg/mL),中药组小鼠灌胃中药提取液(1.0 g/mL),发酵中药组小鼠灌胃发酵中药提取液(1.0 g/mL),模型组和空白组小鼠灌胃生理盐水,每天定时灌胃给药1次(0.2 mL/只),连续灌胃给药6 d。每日记录小鼠腹泻、体重、采食量等情况;末次给药12 h后采集小鼠心脏、肝脏、肾脏、脾脏、肺脏组织,称重并统计脏器系数;收集小鼠肠道内容物进行菌落计数。【结果】试验成功构建大肠杆菌性腹泻小鼠模型。与空白组相比,模型组小鼠腹泻率为100%,体重、平均日增重、平均日采食量、肠道中乳酸菌数量均极显著下降(P<0.01),肝脏指数、脾脏指数以及肠道中大肠杆菌和沙门菌数量均极显著升高(P<0.01)。与模型组相比,抗生素和中药组小鼠腹泻率均为30%,发酵中药组小鼠腹泻率为0;抗生素组小鼠终末体重、平均日增重和平均日采食量均极显著升高(P<0.01),肝脏指数、脾脏指数以及肠道中大肠杆菌和沙门菌数量均极显著下降(P<0.01);中药和发酵中药组小鼠终末体重、平均日增重、平均日采食量和肠道中乳酸菌数量均极显著升高(P<0.01),料重比、肝脏指数和脾脏指数、肠道中大肠杆菌和沙门菌数量均极显著下降(P<0.01)。【结论】糙皮侧耳菌HXS-M1发酵复方中药可降低大肠杆菌性腹泻小鼠的腹泻率,增加平均日采食量和平均日增重,降低肝脏指数和脾脏指数以及肠道中大肠杆菌和沙门菌数量,提高乳酸菌数量,从而对大肠杆菌性小鼠腹泻产生协同保护作用,试验结果可为开发防治大肠杆菌感染致腹泻产品提供参考。

【Objective】The objective of this experiment was to study the clinical therapeutic effect of fermented compound traditional Chinese medicine of Pleuron ostreatus HXS-M1 on Escherichia coli-induced diarrhea in mice,and provide a new therapeutic method for clinical Escherichia coli diarrhea.【Method】The diarrhea model of mice was established by intraperitoneal injection of Escherichia coli suspension(0.2 mL/mouse).40 mice with diarrhea were randomly divided into model group,antibiotic group(AB),traditional Chinese medicine group(TCM)and fermented traditional Chinese medicine group(FTCM),with 10 mice in each group.Another 10 healthy mice were assigned to the blank group with intraperitoneal injection of normal saline(0.2 mL/mouse).Mice in AB group were given enrofloxacin solution(50 mg/mL),mice in TCM group were given traditional Chinese medicine extract(1.0 g/mL),mice in FTCM group were given fermented traditional Chinese medicine extract(1.0 g/mL),mice in model and blank groups were given normal saline intragastric administration once a day(0.2 mL/mouse).The drug was given by continuous intragastric administration for 6 days.The diarrhea,body weight and feed intake of mice were recorded daily.The heart,liver,kidney,spleen and lung tissues of mice were collected 12 h after the last administration,weighed and organ coefficients were calculated.The intestinal contents of mice were collected for colony counting.【Result】The mouse model of Escherichia coli diarrhea was successfully constructed.Compared with blank group,the diarrhea rate of model group was 100%,body weight,average daily gain,average daily feed intake and intestinal lactobacillus number were extremely significantly decreased(P<0.01),liver index,spleen index and intestinal Escherichia coli and Salmonella number were extremely significantly increased(P<0.01).Compared with model group,the diarrhea rate of AB and TCM groups was 30%,and the diarrhea rate of FTCM group was 0.In AB group,the final body weight,average daily gain and average daily feed intake were extremely significantly increased(P<0.01),while liver index,spleen index and the number of Escherichia coli and Salmonella in intestinal were extremely significantly decreased(P<0.01).The final body weight,average daily gain,average daily feed intake and intestinal lactobacillus quantity in TCM and FTCM groups were extremely significantly increased(P<0.01),while the ratio of feed to gain,liver index and spleen index,intestinal Escherichia coli and Salmonella quantity were extremely significantly decreased(P<0.01).【Conclusion】The fermented compound traditional Chinese medicine of Pleurotus ostreatus HXS-M1 could reduce the diarrhea rate,increase the average daily feed intake and average daily gain,reduce the liver index and spleen index,and the number of Escherichia coli and Salmonella in intestine,and increase the number of lactic acid bacteria,so as to have a synergistic protective effect on Escherichia col i diarrhea in mice.The results provided reference for the development of prevention and treatment of diarrhea caused by Escherichia col i infection.