PDA@Ag-Amoxicillin复合材料的合成及协同抗菌效果研究

Study on the synthesis and synergistic antibacterial effects of PDA@Ag-amoxicillin composite material

由于抗菌剂的不当使用和微生物的自然选择过程,抗生素耐药性已成为挑战人类健康的一个严重问题。将两种不同的杀菌剂整合到一个基质中是一种提高抗菌效率的可行方法。通过将聚多巴胺修饰的银纳米粒子(PDA@Ag)与阿莫西林(AMOX)共轭,合成了一种高效的抗菌纳米复合材料。利用TEM、UV-Vis和FT-IR等来表示PDA@Ag-AMOX(PAA)的结构。与PDA@Ag相比,PAA对大肠杆菌的抑制率提高了33.3%,对枯草杆菌的抑制率提高了31.3%,对沙门氏菌的抑制率提高了28.6%。据推测,其抗菌机理是PAA中的Ag^(+)与重要酶和蛋白质的官能团反应,以及AMOX对细菌细胞壁合成和结合蛋白的羧肽酶活性的抑制作用。PAA既具有良好的协同抗菌活性,又具有较高的生物相容性,这为今后抗菌剂之间的组合和探索更多的协同治疗方案奠定了基础。

Owing to the inappropriate use of antimicrobial agents and the process of natural selection of microorganisms,antibiotic resistance has become a serious problem that challenges the health of human beings.Integrating two different bactericidal agents into one matrix is a feasible approach to enhance antibacterial efficiency.This study synthesized an efficient antibacterial nanocomposite material by conjugating polydopamine-modified silver nanoparticles(PDA@Ag)with amoxicillin(AMOX).The structure of PDA@Ag-AMOX(PAA)was characterized using TEM,UV-Vis,and FT-IR.Compared to PDA@Ag,PAA showed an increased inhibition rate of 33.3%against Escherichia coli,31.3%against Bacillus subtilis,and 28.6%against Salmonella.It was speculated that the antimicrobial mechanism involved the reaction of Ag^(+)derived from PAA with functional groups of vital enzymes and proteins and the inhibitory effect of AMOX on the synthesis of bacterial cell walls and carboxypeptidase activity of binding proteins.PAA has both good coordinated antibacterial activity and high biocompatibility,which lays the foundation for the future combination and the exploration of more collaborative treatment plans among antibacterial agents.