荧光探针法测定甲基苯丙胺的方法建立及机理研究

Study on Host-guest Interaction of Methamphetamine with Cucurbit[7]uril by Fluorescent Probe

甲基苯丙胺是一种强效兴奋剂,滥用后具有强烈的成瘾性和神经毒性。以巴马汀为荧光探针,通过紫外分光光度法、荧光分光光度法、分子模拟理论计算以及核磁共振光谱法研究甲基苯丙胺与巴马汀探针对葫芦[7]脲空腔的竞争包合反应。巴马汀-葫芦[7]脲探针体系在水溶液中具有强荧光,加入甲基苯丙胺会猝灭体系的荧光。研究显示,在0.05μg/mL~1μg/mL浓度范围内,甲基苯丙胺与探针体系荧光强度的变化值ΔF呈现良好的线性关系,据此建立了测定甲基苯丙胺的荧光光谱分析方法,方法检测限为6ng/mL。该方法具有灵敏度高、选择性好以及操作简便等优良特性,可用于尿液中甲基苯丙胺的含量测定。

Methamphetamine(MA)is a powerful stimulant drug of abuse with potent addictive and neurotoxic properties.The competitive reaction of MA with palmatine(PAL)fluorescent probe for the occupancy of the cucurbit[7]uril(CB[7])cavity was studied using absorption,spectrofluorimetry,molecular modeling theoretical calculations,and 1 H NMR.The PALCB[7]complex exhibited high fluorescence in aqueous solution,which was quenched in the presence of MA.The fluorescence quenching values(ΔF)showed good linear relationship with MA concentrations from 0.05 to 1μg/mL with a detection limit of 6 ng/mL.Based on the significant quenching of the complex fluorescence intensity,a simple spectrofluorimetric method with high sensitivity and selectivity was developed to determine the content of MA in human urine.