铝试剂在共振光散射光谱法测定氯霉素含量中的应用研究

Study on the Application of Aluminon in the Determination of Chloramphenicol by Resonance Light Scattering Spectrometry

本文以铝试剂为基础,通过氢键相互作用构建了铝试剂-氯霉素超分子体系,建立了超分子体系共振光散射强度变化与氯霉素浓度的定量关系,进而实现氯霉素含量测定。详细考察了实验条件的影响,结果显示,铝试剂浓度为5.0×10^(-4)mol/L、体积为1.5mL、盐酸用量2.5mL、氯霉素浓度和体积分别为100μg/mL和4.0mL时,超分子体系的共振光散射减弱程度最大,此时超分子中氯霉素和铝试剂的摩尔比为2∶1。该方法在8~40μg/mL范围内线性良好,相关系数R2为0.9906,检出限为0.01420μg/mL。在氯霉素加标浓度为8、10、12μg/mL时对方法的准确度进行了考察,平均回收率分别为100.6%、100.1%和100.6%,相对标准偏差(RSD,n=5)分别为1.07%、0.68%和0.75%。该方法应用于氯霉素滴眼液的含量测定,测得标示量百分含量平均值为100.1%,RSD(n=6)为0.74%。

Resonance light scattering spectroscopy is widely used in the field of drug analysis due to its high sensitivity,simple operation,and low cost.In this experiment,the aluminonchloramphenicol supermolecule system was constructed through hydrogen bond interaction,and the quantitative relationship between resonance light scattering intensity and the concentration of chloramphenicol was established,so as to realize the content determination of chloramphenicol.The influence of experimental conditions was investigated in detail,and the results showed that the concentration of aluminon was 5.0×10^(-4)mol/L,the volume was 1.5 mL,the dosage of hydrochloric acid was 2.5 mL,and the concentration and volume of chloramphenicol were 100μg/mL and 4.0 mL,the resonance light scattering of supermolecule system was the most weakened,and the molar ratio of chloramphenicol and aluminon was 2∶1(mol/mol).This method exhibited good linearity in the concentration range of 8-40μg/mL(y=156.26x+3919.6,R 2=0.9906),with a detection limit of 0.01420μg/mL.The accuracy of chloramphenicol at the spiked samples of 8,10,12μg/mL was evaluated,with average recoveries of 100.6%,100.1%and 100.6%,and RSD%of 1.07%、0.68%and 0.75%,respectively(n=5).Finally,the method was successfully applied to the content determination of chloramphenicol eye drops,and the average content was 100.1%,RSD%was 0.74%(n=6).The above results showed that the resonance light scattering spectrometry established could be used for the quantitative analysis of chloramphenicol.