胀包学生饮用乳中不解糖嗜胨菌的鉴定

Identification of Peptoniphilus asaccharolyticus Isolated from Spoiled Fermented Milk Intended for Students

以学生饮用乳(发酵乳)来源的不解糖嗜胨菌为研究对象,基于分离计数、形态学观察、生化鉴定、基质辅助激光解吸电离飞行时间质谱(matrix-assisted laser desorption ionization-time-of-flight mass spectrometry,MALDITOF MS)和全基因组测序技术,建立适用于不解糖嗜胨菌的鉴定方法。结果表明:不解糖嗜胨菌在PCA培养基上厌氧环境下生长较适;在全自动微生物鉴定系统上鉴定为不解糖嗜胨菌,置信度98%;MALDI-TOF MS鉴定为不解糖嗜胨菌,鉴定比对值为2.241。通过全基因组测序,平均核苷酸一致性分析结果为98.58%。利用本研究建立的方法能够满足不解糖嗜胨菌的快速、准确鉴定,符合食品安全检测需求。

This study focuses on the identification of Peptoniphilus asaccharolyticus from spoiled fermented milk intended for students based on isolation,counting,morphological observation,biochemical identification,matrix-assisted laser desorption ionization time-of-flight mass spectrometry(MALDI-TOF MS),and whole-genome sequencing.The results showed that the isolate grew well on PCA medium under anaerobic conditions and was identified as P.asaccharolyticus on a fully automated microbial identification system with a confidence level of 98%.By MALDI-TOF MS,it was confirmed as P.sasaccharolyticus with a comparison value of 2.241.The result of whole genome sequencing showed an average nucleotide identity(ANI)of 98.58%.The proposed method allows rapid and accurate identification of P.sasaccharolyticus and can meet the requirements of food safety detection.