基于生物信息学分析肺腺癌潜在预后生物标记物HROB

Analysis of Potential Prognostic Biomarker HROB in Lung Adenocarcinoma Based on Bioinformatics

目的:通过生物信息学方法探讨HROB在肺腺癌(LUAD)中的表达及预后相关性。方法:从TCGA数据库下载LUAD患者的基因表达谱和临床数据,从HPA数据库下载LUAD患者及健康人群免疫组织化学数据,运用R软件分析HROB基因在LUAD和癌旁组织中的表达差异。临床相关性分析揭示HROB与LUAD患者临床病理特征的相关性。Kaplan-Meier生存分析结合ROC曲线评估HROB的诊断价值及预后分析。Cox回归分析寻找LU⁃AD独立预后因素。GO/KEGG及GSEA信号通路富集分析HROB在LUAD中的潜在功能。CIBERSORT评估HROB表达水平与肿瘤浸润性免疫细胞的相关性。结果:HROB在LUAD中的表达高于癌旁正常组织(P<0.05),并与年龄、吸烟情况、初始治疗效果、TNM分期、病理分级、总生存期(OS)、疾病特异性生存期(DSS)和无进展间隔期(PFI)等临床病理特征呈正相关(P<0.05)。生存分析表明HROB低表达的LUAD患者OS、PFI、DSS明显高于高表达患者(P<0.05)。Cox回归分析发现病理分级Ⅲ、Ⅳ级是影响LUAD预后的独立危险因素(P<0.05),HROB高表达尚不能视为独立预后因子(P>0.05)。功能富集分析提示HROB主要参与细胞周期、染色体分离和同源重组等相关通路。在LUAD免疫细胞浸润中,HROB高表达与NK CD56dim细胞、Tgd细胞、Th2细胞呈正相关(P<005)。结论:HROB的高表达与LUAD患者的进展、预后和免疫调节密切相关,它可能成为LUAD预测预后的生物标志物和治疗靶点。

Objective To analyze the expression of HROB in lung adenocarcinoma(LUAD)and its correlation with progno⁃sis based on bioinformatics.Methods Gene expression profiles and clinical data of LUAD patients were downloaded from the TCGA database.The immunohistochemical data of LUAD patients and healthy individuals were obtained from the HPA database.R software was used to analyze the differential expression of HROB between tumor and adjacent tissues.Clinical correlation analysis revealed the association between HROB and clinicopathological features of LUAD patients.Kaplan-Meier survival analysis combined with ROC curve was employed to evaluate the diagnostic value and prognosis of HROB.Cox regression analysis was conducted to identify the independent prognostic factors,and GO/KEGG and GSEA were per⁃formed to explore the potential functions of HROB in LUAD.The correlation between HROB and tumor-infiltrating immune cells was assessed using CIBERSORT.Results HROB was higher in LUAD tissues than in adjacent normal tissues(P<005)and its expression was positively correlated with age,smoking,initial treatment effect,TNM stage,pathological grade,overall survival(OS),disease-specific survival(DSS),and progression-free interval(PFI)(P<0.05).Survival analysis showed that LUAD patients with low HROB had higher OS,PFI,and DSS than those with high HROB(P<005).Cox regression analysis revealed that pathological gradesⅢandⅣwere independent risk factors for the prognosis of LUAD(P<0.05),while high HROB could not be regarded an independent prognostic factor(P>0.05).Function enrichment a⁃nalysis suggested that HROB was primarily involved in cell cycle,chromosome segregation,and homologous recombination.In the immune cell infiltration of LUAD,there was a positive correlation between high HROB and NK CD56dim,Tgd,and Th2 cells(P<0.05).Conclusion High HROB is closely associated with progression,prognosis,and immune regulation of LUAD patients,indicating its potential as a prognostic biomarker and therapeutic target for LUAD.