miR-155-5p对大鼠急性心肌梗死致心肌凋亡的影响研究

Study on the Effect of miR-155-5p on Myocardial Apoptosis Induced by Acute Myocardial Infarction in Rats

目的本研究探讨了miR-155-5p在大鼠急性心肌梗死模型中的保护作用。方法通过向健康雄性SD大鼠注射空载体和miR-155-5p腺病毒,分为空载体模型组和miR-155-5p过表达组,研究其对心脏功能、心肌损伤指标和心肌细胞凋亡的影响。结果结果显示,miR-155-5p过表达组的心脏功能显著改善,心肌损伤标志物降低,心肌梗死面积和细胞凋亡减少。进一步发现miR-155-5p过表达也能增强H9c2心肌细胞在缺氧条件下的存活能力,降低凋亡相关蛋白的表达。结论这表明miR-155-5p的过表达可以有效保护心肌细胞,减轻急性心肌缺血引起的心肌损伤,有望成为心肌梗死治疗的新靶点。此研究为miR-155-5p在心肌梗死诊断和治疗中的应用提供了新的理论依据。

Objective This study investigates the protective role of miR-155-5p in rat models of acute myocardial infarction.Methods Healthy male SD rats were injected with either control vector or miR-155-5p adenovirus,forming control model groups and miR-155-5p overexpression groups,to study the effects on cardiac function,myocardial injury markers,and myocardial cell apoptosis.Results The results showed that the cardiac function of the miR-155-5p overexpression group significantly improved,myocardial injury markers were reduced,and the area of myocardial infarction and cell apoptosis were decreased.Additionally,overexpression of miR-155-5p also enhanced the survival ability of H9c2 cardiomyocytes under hypoxic conditions and reduced the expression of apoptosis-related proteins.Conclusion This indicates that the overexpression of miR-155-5p can effectively protect myocardial cells and alleviate myocardial damage caused by acute myocardial ischemia,potentially serving as a new target for the treatment of myocardial infarction.This study provides a new theoretical basis for the application of miR-155-5p in the diagnosis and treatment of myocardial infarction.