3D打印钛合金多孔支架表面构建羟基磷灰石涂层促进骨生长

Construction of Hydroxyapatite Coating on Porous Scaffold Surface of 3D Printed Titanium Alloy to Promote Bone Growth

为了解决人工关节假体的应力屏蔽问题,基于3D打印技术设计了70%孔隙率的X型多孔钛合金支架,并在支架表面构建羟基磷灰石(HA)涂层,探究了反应时间及不同预处理方法对HA涂层沉积效果的影响。发现水热反应12 h,且经酸蚀+预碱化+预钙化处理后HA涂层效果最好。将涂覆HA涂层的X型结构多孔Ti6Al4V合金支架与小鼠的MC3T3-E1细胞共同培养,评价其细胞毒性、生物相容性、细胞增殖行为以及成骨能力。结果表明,HA涂层可以增强多孔Ti6Al4V合金支架的生物相容性,使得MC3T3-E1细胞在多孔Ti6Al4V合金支架上更易于粘附和铺展,有利于钙、磷等元素的沉积,使支架矿化能力增强,骨整合能力提高。

In order to solve the stress shielding problem of artificial joint prosthesis,an X-type porous titanium alloy scaffold with 70%porosity was designed based on 3D printing technology,and a hydroxyapatite(HA)coating was constructed on the surface of scaffold.The effects of reaction time and different pretreatment methods on the deposition effect of the HA coating were investigated.It is found that the HA coating has the best effect after 12 h of hydrothermal reaction,and acid etching,pre-alkalinization,and pre-calcination treatments.The X-shaped porous Ti6Al4V alloy scaffold with HA coating is co-cultured with mouse MC3T3-E1 cells to evaluate their cytotoxicity,biocompatibility,cell proliferation,and osteogenic ability.The results show that the HA coating can enhance the biocompatibility of porous Ti6Al4V alloy scaffold,making it easier for MC3T3-E1 cells to adhere and spread on the porous Ti6Al4V alloy scaffold.It promotes the deposition of calcium,phosphorus and other elements,thereby enhancing the mineralization ability and improving the osseointegration ability of the scaffold.