转录组测序技术在甘薯研究中的应用

Application of Transcriptome Sequencing Technology in Sweetpotato(Ipomoea batatas L.)Research

甘薯是世界上重要的粮食、饲料、工业原料作物。随着高通量测序技术的快速发展,转录组测序技术已广泛应用于甘薯研究之中。为了今后利用转录组学技术更好地开展甘薯功能基因挖掘、重要性状调控机制解析等研究,本综述分析了各国研究机构共享的甘薯转录组数据,并总结了近年来转录组测序技术在甘薯逆境胁迫响应、块根发育和淀粉合成等方面的应用及取得的成果。认为目前甘薯转录组研究在试验设计、数据挖掘以及研究结果实用性方面仍存在不足。提出在未来研究中,可将转录组学技术与其他组学技术及遗传学分析方法相结合,以推动甘薯研究的不断深入。

Sweetpotato is an important food,fodder and industrial raw material crop in the world.With the rapid development of high-throughput sequencing technologies,transcriptome sequencing technology has been widely used in sweetpotato studies.To better carry out research on functional gene mining and important trait regulation mechanisms analyzing in sweetpotato using transcriptomics techniques in the future,this study analyzed the profile of sweetpotato transcriptome data shared by research institutions in various countries,and summarized the applications and achievements of transcriptome sequencing technology in researches on sweetpotato over recent years,from the aspects of response to adversity stress,tuberous root development and starch synthesis.It was pointed out that there were still deficiencies in experimental design,data mining,and practicality of research results in current sweetpotato transcriptome studies.It was proposed that in further studies,transcriptomics technologies should be integrated with other omics technologies and genetic analysis methods to promote the continuous deepening of sweetpotato research.