不同生物饵料对中国鲎繁殖性能及后代质量的影响

Effects of Different Biological Diets on Reproductive Performance and Offspring Quality of Horseshoe Crab(Tachypleus tridentatus)

为筛选有效促进中国鲎(Tachypleus tridentatus)亲鲎性腺发育及产卵的生物饵料,选取成年中国鲎15对,于2021年7—8月开展生物饵料实验,以菲律宾蛤仔(A组)、双齿围沙蚕(B组)、近江牡蛎肉(C组)、菲律宾蛤仔+双齿围沙蚕(D组)和近江牡蛎肉+双齿围沙蚕(E组)5组生物饵料投喂亲鲎,采用SPSS 19.0软件统计分析和单因素方差分析,通过比较卵径、受精卵的孵化时间和孵化率以及1龄幼体头胸甲宽,研究不同饵料对中国鲎繁殖性能和后代质量的影响。结果表明:试验D组产卵数量和次数均高于其他试验组,其次是E组,混合试验组产卵数量和质量均高于单独试验组。研究发现,双齿围沙蚕和菲律宾蛤仔组合投喂较适合开发为亲鲎性腺强化培育的生物饵料,对性腺发育及产卵有显著的促进作用。本研究旨在为开发安全、高效、优质的中国鲎性腺促熟饵料提供参考依据。

The aim of this study is to identify biological diets that effectively enhance gonadal development and spawning in T.tridentatus.Five groups of biological diets were administered to T.tridentatus,comprising Ruditapes philippinarus(A),Perinereis bidentata(B),Ostreagigas(C),R.philippinarus+P.bidentata combination(D),andOstreagigas+P.bidentata combination(E).The effects of different diets on the quality of offspring were analyzed by comparing the egg diameter,hatching rate and the width of the first-age larva's cuticle,and the effects of different diets on reproductive performance were evaluated.The results showed that the number and frequency of spawning in group D were higher than those of other test groups,followed by group E,and the number and quality of spawning in mixed test group were higher than those of single test group.R.philippinarus and P.bidentata are more suitable for the development of parent T.tridentatus gonadal enhanced culture,which can significantly promote the gonadal development and spawning.The conclusion of this study provides reference for the development of safe,efficient and high-quality gonadal ripening food for T.tridentatus.