全细胞转化生产D-阿洛酮糖条件优化

Optimization of Conditions for Whole Cell Transformation to Produce D-allulose

全细胞体系制备D-阿洛酮糖因成本低、工艺简单受到人们的关注,研究全细胞转化D-果糖为D-阿洛酮糖的较适条件对促进D-阿洛酮糖的产业化具有重要意义。该文探究了全细胞酶的储藏稳定性并对全细胞制备D-阿洛酮糖的条件(培养时间、细胞浓度、转化初始pH、转化温度、金属离子)进行了优化与验证。结果表明:全细胞酶具有较强的储藏稳定性,40℃放置48 h催化效果未明显降低;在500 g/L D-果糖底物浓度下,适用于生产的全细胞转化较适条件为细胞浓度10 g/L、转化温度70℃、转化初始pH为自然pH、转化率为31.55%,较优化前转化率提高约3%,转化周期缩短至4 h,生产工序简化、生产效率提高、生产成本降低。该研究旨在为D-阿洛酮糖工业化生产提供实验基础,满足大规模生产的需求。

The production of D-allulose in a whole cell system has attracted attention due to its low cost and simple process.Studying the suitable conditions for the conversion of D-fructose into D-allulose in whole cells is of great significance in promoting the industrialization of D-allulose.This article explored the storage stability of whole cell enzymes as well as optimized and validated the conditions for the production of D-allulose from whole cells(including culture time,cell concentration,initial conversion pH,conversion temperature,and metal ions).The results showed that the whole cell enzyme had relatively strong storage stability,and the catalytic effect did not significantly decrease when placed at 40℃for 48h;At a concentration of 500g/L D-fructose substrate,the suitable conditions for whole cell transformation in production were cell concentration of 10 g/L,conversion temperature of 70℃,initial conversion pH equaling to natural pH,and conversion rate up to 31.55%,which was about 3%higher than before optimization.The conversion cycle was shortened to 4 h,and the production process was simplified,production efficiency was improved,and production costs were reduced.The research aims to provide an experimental basis for the industrial production of D-allulose and meet the needs of large-scale production.