参芪复合固体饮料中3种有效成分检测方法的建立及其含量稳定性的研究

Study on the Establishment of Detection Methods and Content Stability of Three Effective Components in Shenqi Compound Solid Beverage

通过HPLC测定参芪复合固体饮料中人参皂苷Rg1、甘草酸、黄芪甲苷的含量,并进行加速试验考察其稳定性。结果表明,人参皂苷Rg1、甘草酸、黄芪甲苷分别在3.1709~63.4186μg/mL、0.7945~15.8993μg/mL、20.3199~203.1993μg/mL浓度范围内线性良好,加标回收率范围分别为100.27%~104.87%、100.74%~104.72%、103.07%~107.54%。在3个月加速试验中,该固体饮料性状、感官、溶解度均符合要求;3种成分含量均呈现出良好的稳定性。表明参芪复合固体饮料中人参皂苷Rg1、甘草酸、黄芪甲苷的含量在3个月加速试验中相对稳定,该研究可为参芪复合固体饮料的有效期制定提供良好依据。

HPLC was used to determine the content of ginsenoside Rg1,glycyrrhizic acid,and astragaloside Ⅳ in Shenqi compound solid beverage,and their stability was investigated through accelerated experiment.The results showed that ginsenoside Rg1,glycyrrhizic acid,and astragaloside Ⅳ had a good linear relation in the respective concentration range of 3.1709~63.4186μg/mL,0.7945~15.8993μg/mL and 20.3199~203.1993μg/mL,the range of sample recovery rates were 100.27%~104.87%,100.74%~104.72%,103.07%~107.54%,respectively.3-month accelerated experiment showed that textural properties,sensory properties,and solubility of the solid beverage met the requirements;The content of all three components showed good stability.This indicates the stability of ginsenoside Rg1,glycyrrhizic acid,and astragaloside Ⅳ in Shenqi compound solid beverage was good in 3-month accelerated experiment,this study provides a good reference for determining the expiration date of Shenqi compound solid beverage.