敖汉细毛羊羊毛经济性状的全基因组关联分析

Genome-Wide Association Study of Wool Economic Traits in Aohan Fine Wool Sheep

旨在利用全基因组关联分析探寻敖汉细毛羊羊毛性状新的分子标记和候选基因。本研究采集1~2周岁的健康敖汉细毛羊耳组织与羊毛作为试验素材,其中,母羊248只,公羊81只,总计329只。羊毛进行性状测定(包括纤维直径、自然长度、伸直长度、伸直率),并对表型数据进行描述性统计和相关性分析。利用绵羊40K液相SNP芯片对全部个体进行基因分型。使用Plink 1.07软件对芯片数据进行质控,使用GCTA软件和PopLDdecay软件对质控数据进行群体结构分析。利用GMEMA混合线性模型对4种羊毛性状进行了全基因组关联分析(genomewide association study,GWAS),利用在线软件对候选基因进行GO和KEGG富集分析。质控后得到329只个体的30079个SNPs位点用于后续分析。通过GWAS分析筛选出4个在全基因组上显著相关的SNPs位点可能影响羊毛经济性状,分别位于1号、6号及8号染色体上。筛选出9个在染色体水平上显著相关的SNPs位点可能对羊毛性状具有潜在意义,分别位于3、5、8、11、18、21、22、25号染色体,寻找到39个可能影响羊毛性状的候选基因。本研究结果为后续探究敖汉细毛羊羊毛性状的遗传机制及分子育种标记开发提供重要参考。

The study aimed to identify new molecular markers and candidate genes for wool traits in Aohan fine wool sheep using genome-wide association study.The ear tissue and wool of 1-2 years old healthy Aohan fine wool sheep were collected as experimental materials,248 ewes and 81 rams,totaling 329.The wool traits were detected(including fiber diameter,natural length,elongation length,and elongation ratio),and the phenotypic data were subjected to descriptive statistics and correlation analysis.All individuals were genotyped using the 40K liquid phase SNP chip for sheep.The chip data were quality controlled using PLINK 1.07 software,and the qualitycontrolled data were analyzed for population structure using GCTA software and PopLDdecay software.The GMEMA mixed linear model was used to perform a genome-wide association study(GWAS)on 4 wool traits,and online software was used to conduct GO and KEGG enrichment analyses on candidate genes.After quality control,329 individuals with 30079 SNP loci were used for subsequent analysis.Through GWAS analysis,4 SNP loci were identified as significantly associated with wool economic traits across the genome,located on chromosomes 1,6,and 8.Additionally,9 SNP loci were identified as significantly associated with wool traits at the chromo-some level,located on chromosomes 3,5,8,11,18,21,22,and 25,and 39 candidate genes were identified as potentially affecting wool traits.The results of this study provide important references for further exploring the genetic mechanisms and molecular breeding markers of wool traits in Baohan fine wool sheep.