盐胁迫下木榄根系木栓化分子调控机制研究

Molecular mechanisms of suberinization in the roots of Bruguiera gymnorrhiza subjected to salt stress

选择木榄幼苗作为实验对象,利用转录组测序和实时荧光定量PCR技术分析盐胁迫下木榄幼苗根系木栓化过程与分子调控机制。结果表明,盐分胁迫后共获得35561个差异表达基因,包括36351个上调基因, 210个下调基因。GO注释表明差异表达基因主要集中在细胞氮化合物代谢过程、细胞和细胞组分、以及结构分子活性等过程中。KEGG代谢通路注释表明,差异表达基因显著富集到核糖体和氧化磷酸化途径,同时发现拖品烷,哌啶,聚酮生物碱的生物合成也被显著富集。我们进一步分析得到了39个可能与木栓质单体合成、跨膜转运及聚合相关的基因。另外, qRT-PCR分析结果同样表明长链酯酰辅酶A合成酶1基因、谷胱甘肽转移酶(GST)和MYB转录因子(MYB)在盐胁迫后上调表达。本研究为红树植物耐盐机制以及木栓质生物合成分子调控机制提供基础数据,同时也对红树林的保护与恢复有重要意义。

RNA-Seq(RNA sequencing)and qRT-PCR(Quantitative Real-time PCR)were utilized to elucidate the molecular mechanisms involved in suberin biosynthesis in the roots of Bruguiear gymnorrhiza in response to salt stress.A total of 36561 DEGs(differentially expressed genes)were identified,including 36351 upregulated genes and 210 downregulated genes.GO(Gene Ontology)annotation showed that these DEGs were primarily associated with cell nitrogen compound metabolism,cell and cell composition,and structural molecule activity.KEGG(Kyoto Encyclopedia of Genes and Genomes)metabolic pathway annotation indicated that the differentially expressed genes were significantly enriched in ribosomes and oxidative phosphorylation.We also observed significant enrichment in tropane,piperidine,and polyketide alkaloid biosynthesis pathways.Furthermore,39 genes related to suberinization,such as monomer synthesis,transmembrane transport and polymerization,were also identified.Nevertheless,qRT-PCR results also showed that long chain acyl-CoA synthetase 1(LACS1),glutathione S-transferase(GST)and the Myb superfamily(MYB)were upregulated under salt stress.This study provides fundamental insights into the mechanism of salt tolerance associated with suberinization and may have significant implications for mangrove protection and restoration.