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共聚焦显微镜线性扫描控制系统设计

The Design of Linear Scanning Control System for Confocal Microscope
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摘要 为了实现共聚焦显微镜系统中的振镜线性扫描,并改善振镜噪声及系统稳定性,设计了基于FPGA的振镜扫描控制系统。该系统通过FPGA生成振镜扫描信号,输出信号幅值、频率精准,通过ARM芯片与上位机通讯,实现振镜系统的扫描控制。在扫描波形上,通过对三角波端点处进行三次样条插值处理,使振镜扫描过程中速度及加速度连续,提高振镜运行稳定性。在输出波形上通过累加器进行频率控制,提高了频率分辨率。实验表明,设计的振镜扫描控制系统波形、频率及幅值准确,成像效果清晰、细腻,并可有效降低振镜噪声。 In order to realize the linear scanning of the galvanometer in the confocal microscope system and improve the noise of the galvanometer and the stability of the system,a galvanometer scanning control system based on FPGA is designed.The system generates the galvanometer scanning signal through FPGA,and the output signal amplitude and frequency are accurate.It communicates with the host computer through the ARM chip to realize the scanning control of the galvanometer system.On the scanning waveform,cubic spline interpolation is performed on the endpoints of the triangular wave to make the speed and acceleration continuous during the scanning process of the galvanometer and improve the operational stability of the galvanometer.Frequency control is performed through the accumulator on the output waveform,and the frequency resolution is high.The galvanometer scanning control system designed in this article is compact in size,which can effectively improve the integration of the confocal microscope system and reduce costs.
作者 何大永 赵春华 王振亚 张瀚文 胡金田 HE Dayong;ZHAO Chunhua;WANG Zhenya;ZHANG Hanwen;HU Jintian(School of Life Science and Technology,Changchun University of Science and Technology,Changchun 130022;Suzhou Institute of Biomedical Engineering and Technology,Chinese Academy of Sciences,Suzhou 215163)
出处 《长春理工大学学报(自然科学版)》 2024年第5期65-70,共6页 Journal of Changchun University of Science and Technology(Natural Science Edition)
基金 中国科学院科研仪器设备研制项目(ZDZBGCH2018003)。
关键词 共聚焦显微镜 振镜扫描 FPGA confocal microscope galvanometer scanning FPGA
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