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胰腺神经内分泌肿瘤患者舌苔菌群微生态分析

Microecological analysis of tongue coating microbiota in patients with pancreatic neuroendocrine neoplasms
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摘要 目的探讨胰腺神经内分泌肿瘤(pNENs)患者舌苔菌群微生态的特点。方法选取2019年11月至2020年7月间南京市第一医院15例经病理确诊的pNENs患者的舌苔菌群,招募15名健康志愿者作为正常对照组。使用口腔咽拭子刮取舌苔,对两组样本舌苔进行DNA提取和检测,通过16S rRNA高通量测序技术比较两组舌苔菌群的差异。分别用Chao1指数、Shannon指数和主成分分析检验α和β多样性差异。采用组间差异分析比较两组间舌苔菌群门、属、种水平上的物种组成,线性判断分析(LEfSe)两组优势菌属的差异。结果pNENs患者与健康对照者舌苔菌群的种类和数量相似,但结构组成明显不同。在门水平上,两组舌苔菌群均以变形菌门(Proteobacteria)、拟杆菌门(Bacteroides)、厚壁菌门(Firmicutes)、梭杆菌门(Fusobacteria)和放线菌门(Actinobacteria)为主,pNENs患者厚壁菌门、放线菌门相对丰度较高,而健康者拟杆菌门较高。在属水平上,两组舌苔菌群菌属均以嗜血杆菌属(Haemophilus)、奈瑟菌属(Neisseria)、普雷沃菌7属(Prevotella_7)、链球菌属(Streptococcus)、梭杆菌属(Fusobacterium)、拟普雷沃菌属(Alloprevotella)、韦荣球菌属(Veillonella)为主,pNENs患者韦荣球菌属相对丰度较高,而健康者梭杆菌属、拟普雷沃菌属、普雷沃菌属(Prevotella)和凝聚杆菌属(Aggregatibacter)相对丰度较高。LEfSe结果显示,pNENs患者优势菌属在门水平和属水平分别为放线菌门和罗氏菌属,健康者在属水平上的优势菌属为凝聚杆菌属。结论pNENs患者舌苔菌群的结构组成与健康人群存在差异,放线菌门及罗氏菌属丰度的增加和凝聚杆菌属丰度的减少可能对pNENs的诊断存在潜在的意义。 ObjectiveTo explore the characteristics of tongue microbiota in patients with pancreatic neuroendocrine neoplasms(pNENs).MethodsThe tongue coating microbiota of 15 pathologically diagnosed pNENs patients from Nanjing First Hospital between November 2019 and July 2020 were selected,and 15 healthy volunteers were recruited as the healthy control group.Oral pharyngeal swab was used to scrape the tongue coating,and DNA extraction and detection of the tongue coating samples from two groups were performed,16S rRNA gene high-volume sequencing was applied to analyze the differences on the microbiome.Theαandβdiversity differences were tested by Chao1 index,Shannon index and principal component analysis,respectively.The inter-group analysis of variances was used to compare the species composition of tongue coating microbiota at the phylum,genus,and species levels between two groups.Differences in dominant bacterial genera between two groups were analyzed by using linear discriminant analysis(LEfSe).ResultsCompared with the healthy control group,the types and quantities of tongue coating microbiota in pNENs patients were similar,but the structural composition was significantly different.At the phylum level,Proteobacteria,Bacteroides,Firmicutes,Fusobacteria and Actinobacteria were mainly present in both two groups,but Firmicutes and Actinobacteria were relatively more in pNENs patients compared with healthy groupy with more Bacteroides.The dominant genera of tongue coating microbiota in pNENs patients and healthy individuals included Haemophilus,Neisseria,and Prevotella_7,Streptococcus,Fusobacterium,Alloprevotella and Veillonella.The relative abundance of Fusobacterium,Alloprevotella,Prevotella and Aggregatibacter in the healthy control group's tongue coating microbiota was higher,while Veillonella had a higher relative abundance in the tongue coating microbiota of pNENs patients.LEfSe results showed that the dominant microflora were Actinobacteria at phylum level and Roseburia at genera level in pNENs patients,which were Aggregatibacter at genera level in healthy group.ConclusionsThe distribution of tongue coating microbiota in pNENs patients are different from those in healthy people.The increase in abundance of Actinobacteria and Roseburia,as well as the decrease in abundance of Aggregatibacter,may have potential implications for the diagnosis of pNENs.
作者 王志 吴寒 李超 张振玉 姜宗丹 Wang Zhi;Wu Han;Li Chao;Zhang Zhenyu;Jiang Zongdan(Department of Gastroenterology,Nanjing Medical University Affiliated Nanjing Hospital(Nanjing First Hospital),Nanjing 210006,China;Department of Gastroenterology,Nanjing Jiangbei Hospital,Nanjing 210044,China)
出处 《中华胰腺病杂志》 CAS 2024年第5期345-349,共5页 Chinese Journal of Pancreatology
关键词 胰腺神经内分泌肿瘤 舌苔 菌群 16sRNA基因测序 Pancreatic neuroendocrine neoplasms Tongue coating Microbiota 16S rRNA gene sequencing
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