基于微卫星标记的五垒岛湾绿鳍马面增殖放流效果评估

Evaluation on Stock Enhancement Effect of Thamnaconus septentrionalis in Wuleidao Bay Using Molecular Markers

利用已开发的5对微卫星引物,基于亲子鉴定技术对五垒岛湾绿鳍马面鲀(Thamnaconus septentrionalis)的增殖放流效果进行评估。研究结果显示:200尾繁殖亲本群体和498尾回捕绿鳍马面鲀群体的平均等位基因数(Na)、平均观测杂合度(Ho)、平均期望杂合度(He)和平均多态信息含量(PIC)分别为30.2、0.764、0.819、0.8。亲缘关系结果表明:在父母本性别未知的情况下,5个微卫星位点的非亲权排除率(NEP)和累积非亲权排除率(CEP)分别为0.124~0.405、0.99724280。在回捕的498尾绿鳍马面鲀个体中,27尾回捕个体在模拟分析中被确认为本次增殖放流的子代,因此可以计算出本次增殖放流绿鳍马面鲀对野生群体的贡献率为5.42%。综合分析表明,本研究为后续开展增殖放流效果评估提供科学依据,对绿鳍马面鲀的资源保护以及后续发展科学增殖放流具有重要意义。

Using five polymorphic microsatellite loci based on parentage identificationng technology,the effect of stock enhancement of Thamnaconus septentrionalis in Wuleidao Bay was evaluated.The study results revealed that the average allele number(Na),average observed heterozygosity(Ho),average expected heterozygosity(He),average polymorphic informationcontent(PIC)for the samples(including 200 broodstocks and 498 recaptured individuals)were 30.2,0.764,0.819,0.8,respectively.With the assumption that the gender of the broodstocks is unknown,parentage testing showed that the non-exclusion probability(NEP)and non-exclusion probability(CEP)for five microsatellite loci were 0.124-0.405 and 0.99724280,respectively.Among the 498 captured T.septentrionalis,27 individuals were identified as offsprings in the simulation analysis.Hence,the contribution rate of the stock enhancement to the wild T.septentrionalis resource was 5.42%.In conclusion,the content of this study provided a scientific basis for the subsequent stock enhancement evaluation.Meanwhile,these results are valuable for the subsequent protection and rational exploitation of T.septentrionalis resource.