鼠曲草对大鼠生殖激素水平及免疫机能的影响

Study on the effects of Gnaphalium affine D.Don on reproductive hormone levels and immune function in rats

目的探索鼠曲草(PADA)对大鼠生殖激素水平以及免疫机能的影响。方法将PADA按传统食用习惯提取浓缩后,连续30 d经口给予SD大鼠,对六项生殖激素、免疫器官、碳廓清实验、ConA诱导的脾淋巴细胞转化实验、血清溶血素、抗体生成细胞进行检测。结果暴露PADA后的雌性各剂量组大鼠的催乳素升高(P<0.05);雄性100 g/kg体质量剂量组的脾脏重量、雄性50 g/kg体质量剂量组的血清溶血素抗体积数、雌性100 g/kg体质量剂量组的胸腺重量、胸腺指数升高(P<0.05);碳廓清吞噬指数a、淋转OD差值、溶血空斑数、白细胞、球蛋白、白/球比值、免疫器官的生长发育及组织形态等均未观察到明显的不良改变。结论PADA能引起雌性25 g/kg体质量剂量以上催乳素升高;未观察到免疫功能有明显受损现象;脾、胸腺重量及胸腺指数升高,提示有一定的免疫增强作用。

Objective To study the effects of Pseudognaphalium affine D.Don Anderberg(PADA)on reproductive hormone levels and immune function in rats.Methods PADA was extracted and concentrated according to the traditional eating habit,and given orally to SD rats for 30 consecutive days.Six sex hormones,immune organs,carbon clearance assay,ConA-induced splenic lymphocyte transformation test,determination of serum hemolysin and determination of antibody producing cell were measured.Results The prolactin levels was elevated(P<0.05)in female rats of all dose groups after exposure to PADA.Spleen weight in the male 100 g/kg.bw dose group,antibody product in the male 50 g/kg·bw dose group and thymus weight and thymus-body ratio in the female 100 g/kg·bw dose group were increased(P<0.05).While no significant adverse changes were observed in the phagocytic index-a,the OD difference value of lymphocyte transformation test,Hemolytic plaques,WBC,globulin,the albumin/globulin ratio,and immune organs.Conclusion Prolactin levels can be increased in females at doses above 25 g/kg·bw.No significant impairment of immune function was observed.Spleen weight,thymus weigh,and thymus-body ratio were increased,suggesting a certain immune enhancement effect.