复合益生菌粉对小鼠免疫机能的影响研究

Effect of Compound Probiotic Powder on Immune Function of Mice

目的探究复合益生菌粉在增强机体相关免疫功能的作用,以期为未来更深入地探索和应用该复合益生菌粉提供理论依据和实验支撑。方法将160只ICR小鼠随机分为空白对照组、低、中、高剂量组,采用随机平行对照分组法进行分组,每组各40只。实验周期为30 d,空白组灌胃生理盐水30 d,其余各组按照预设剂量灌胃复合益生菌粉30 d,灌胃结束后,活杀小鼠,观察其脏器指数、迟发性变态反应、淋巴细胞转化、血清溶血素、抗体生成细胞、自然杀伤细胞(nutral killer cells,NK)活性以及巨噬细胞吞噬功能等数据整体性评估复合益生菌粉对小鼠免疫机能的作用。结果相对于空白对照组,各剂量组小鼠体重和脏器指数差异无统计学意义(F=0.885,P=0.458;F=0.844,P=0.479;F=0.732,P=0.540);足趾肿胀程度差值,差异有统计学意义(F=3.252,P=0.03);各剂量小鼠脾淋巴细胞增殖能力光密度差值增加,差异有统计学意义(F=48.786,P=0.000);溶血空斑数增加,差异有统计学意义(F=22.095,P=0.000);中、高剂量组半数溶血值升高,差异有统计学意义(F=30.544,P=0.000);廓清指数和吞噬指数升高,差异有统计学意义且呈剂量依赖性(F=338.79,P=0.000;F=120.271,P=0.000);NK细胞活性同样升高,差异有统计学意义且呈剂量依赖性(F=466.491,P=0.000)。结论复合益生菌粉能提高小鼠免疫力,结果符合《保健食品检验与评价技术规范》中“具有增强免疫力功能”的评价标准。

Objective To explore the effect of compound probiotic powder on enhancing related immune function of organism,so as to provide theoretical basis and experimental support for further exploration and application of compound probiotic powder in the future.Methods 160 ICR mice were randomly divided into control group,low,medium and high dose groups,and divided into groups by random parallel control grouping method,with 40 mice in each group.The experimental period lasts 30 days,the control group was given normal saline for the same time,and the other groups were given compound probiotic powder for 30 days.After the gavage,the mice were killed alive,and their organ index,delayed allergic reaction,lymphocyte transformation,serum hemolysin,antibody-producing cells,NK cell activity and macrophage phagocytosis were observed to evaluate the influence of compound probiotic powder on the immunity of mice.Results Compared with the control group,there was no significant difference in body weight and organ index of mice in each dose group(F=0.885,P=0.458;F=0.844,P=0.479;F=0.732,P=0.540);The difference of toe swelling degree was statistically significant(F=3.252,P=0.03).The difference of optical density of spleen lymphocyte proliferation in mice with different doses increased,and the difference was statistically significant(F=48.786,P=0.000).The number of hemolytic plaque increased with statistical significance(F=22.095,P=0.000).The half hemolysis value of middle and high dose groups increased,and the difference was statistically significant(F=30.544,P=0.000).The clearance index and phagocytosis index increased,and the difference was statistically significant and dose-dependent(F=338.79,P=0.000;F=120.271,P=0.000);NK cell activity also increased,and the difference was statistically significant and dose-dependent(F=466.491,P=0.000).Conclusion The compound probiotic powder can improve the immunity of mice,and the results meet the evaluation standard of"having the function of enhancing immunity"in the Technical Specification for Inspection and Evaluation of Health Food.