不同周龄段雄性长爪沙鼠肾小体的形态变化

Morphological Changes of Renal Corpuscles in Male Mongolian Gerbils at Different Ages

目的通过观察比较不同周龄段的雄性长爪沙鼠的肾脏在最大纵切面上肾小体的变化,初步探索不同周龄段雄性长爪沙鼠肾小体可能存在的变化规律,为选择雄性长爪沙鼠用于肾病相关致病机制及药物筛选等研究提供基础数据支持。方法分别取12周龄、48周龄、72周龄3个周龄段的雄性长爪沙鼠肾脏,从最大冠状面纵切后制成肾脏组织切片;苏木精-伊红染色后,全景扫描成电子组织切片,利用CaseViewer拍摄最大纵切面上肾小体图片,并测量肾小体及其所包含的肾小球、肾小囊面积;使用IBMSPSSStatistics27进行统计分析,使用GraphPad Prism 8制作图表。结果与12周龄相比,48周龄时肾小体(P=0.029)、肾小球(P=0.001)的面积均显著增大,肾小囊面积没有明显变化(P=0.478),肾小球在其肾小体中的面积占比有增大趋势,但差异无统计学意义(P=0.163);与48周龄相比,72周龄时肾小体、肾小球和肾小囊面积均显著增大(P<0.001),但肾小球在其肾小体中的面积占比显著减小(P<0.001)。结论12~72周龄的雄性长爪沙鼠肾小体持续增大,在这个增大过程中可能存在一定规律,即肾小球与肾小体的增大趋势相对一致;而肾小囊与肾小体相比,其增大趋势存在一定滞后性,推测肾小体增大主要是由肾小球增大引起,进而导致肾小囊被动增大造成的;肾小体增大可能是通过多次“肾小球增大–引发肾小囊增大”完成。

Objective By observing and comparing the changes in renal corpuscles at the largest longitudinal section in male Mongolian gerbils of different ages,this study preliminarily explored potential patterns of renal corpuscle changes,providing foundational data for the selection of male Mongolian gerbils for research on the pathogenic mechanisms and drug screening of nephropathy.Methods Kidney samples were collected from male Mongolian gerbils aged 12,48,and 72 weeks.After making longitudinal cuts along the largest coronal plane in the middle,kidney tissue sections were prepared.Following HE staining,panoramic electronic tissue sections were scanned,and images of renal corpuscles at the largest longitudinal plane were captured using CaseViewer.The areas of renal corpuscles and their glomeruli and renal vesicles were measured.Statistical analysis was performed using IBM SPSS Statistics 27,and figures were created using GraphPad Prism 8.Results Compared with the 12-week group,both the area of renal corpuscle(P=0.029)and glomerulus(P=0.001)significantly increased in the 48-week group,while there was no significant change in the area of renal vesicle(P=0.478).The proportion of glomerular area within the renal corpuscle showed an increasing trend but the difference was not statistically significant(P=0.163).Compared to the 48-week group,the areas of renal corpuscle,glomerulus,and renal vesicle were all significantly larger in the 72-week group(P<0.001),but the proportion of glomerular area within renal corpuscle decreased significantly(P<0.001).Conclusion The renal corpuscles in male Mongolian gerbils continued to increase from 12 to 72 weeks of age.There might be a certain pattern in this process of enlargement,where the trends of glomerular and renal corpuscle enlargement were relatively consistent.However,the enlargement of renal vesicles appeared to lag behind that of the renal corpuscle.It was speculated that the enlargement of renal corpuscles was mainly caused by the passive enlargement of renal vesicles due to glomerular enlargement.The enlargement of renal corpuscles might be achieved through multiple cycles of"glomerular enlargement-triggering renal vesicle enlargement".