基因编辑猪-猴异种组织器官移植围手术期动物护理

Perioperative Animal Care for Xenotransplantation from Genetically Edited Pigs to Monkeys

目的探讨基因编辑猪-猴异种组织器官移植围手术期的动物护理,为提高猪-猴异种组织器官移植实验的成功率提供帮助。方法2022年10月—2023年10月,对行基因编辑猪-猴异种组织器官移植的7只受体恒河猴进行围手术期护理和伤口保护。根据体型及手术区域,为受体猴定制伤口防护服以保护伤口,同时做好围手术期精细化护理。其中,术前护理包括术前准备、术前用药等;术中护理包括生理指标监护和麻醉手术护理等;术后护理包括伤口保护、观察监测、营养护理等。结果7只恒河猴的异种组织器官移植手术均获得成功。通过伤口防护服的保护和精细化护理,所有恒河猴手术伤口均一期愈合,术后恢复良好。结论做好猪-猴异种组织器官移植围手术期的护理和伤口保护,不仅能促进伤口愈合,还能减轻手术给动物带来的疼痛和伤害,这对猪-猴异种组织器官移植的实验研究进展和提升动物福利均具有重要意义。

Objective To discuss the perioperative care and wound protection of xenotransplantation from genetically edited pigs to monkeys,with the goal of improving the success rate of such experimental procedures.Methods From October 2022 to October 2023,perioperative care and wound protection were performed on 7 recipient rhesus monkeys undergoing xenotransplantation of genetically edited pig tissues and organs.Customized wound protective garments were designed based on monkeys'size and surgical area to protect the wounds,alongside meticulous perioperative care.This included preoperative preparation and medication,intraoperative monitoring of physiological indicators and anesthesia management,and postoperative care comprising wound protection,observation and monitoring,and nutritional support.Results All seven monkeys successfully underwent xenotransplantation.With the aid of protective garments and detailed care,all surgical wounds healed by first intention,and postoperative recovery was satisfactory.Conclusion Proper care and wound protection during xenotransplantation from genetically edited pigs to monkeys not only promote wound healing,but also alleviate pain and harm to animals.This has significant implications for advancing experimental research in pig-monkey xenotransplantation and enhancing animal welfare.