大肠埃希菌中表达多聚纳米抗体研究进展

Advances in Expression of Multimerized Nanoantibodies(MN)in E.coli

利用大肠埃希菌(Escherichia coli)生产的多聚纳米抗体已经广泛应用于医疗诊断、治疗、食品检测等方面的研究。通过特定多聚体结构域与纳米抗体重组融合从而形成多聚体纳米抗体,具有可提高纳米抗体的稳定性、亲和力、抗水解能力,延长血清半衰期和增加检测灵敏度等优点。本文回顾总结了多聚化纳米抗体的形成机制、类型、功能以及研究的最新进展,综述其在抗肿瘤抗病毒的作用以及作为“探针”诊断疾病、检测食品、环境痕量污染物的应用,为多聚体纳米抗体应用于疾病治疗、诊断、检测试剂研发提供思路。

Multimerized nanoantibodies(MN)produced by E.coli have been widely used in medical diagnosis,treatment,foods detection and other aspects of researches.MN can be formed by recombination and fusion of specific multimolecular domains with nanoantibody,which can improve the stability,affinity,anti-hydrolysis ability and prolong the serum half-life,and improve the detection sensitivity.In this review,the types,formation mechanisms,functions and the latest research progress of MN are summarized.This paper reviews the effects of MN on anti-tumor and anti-virus,as well as their ability as"probes"to diagnose diseases,to detect trace pollutants in the foods and in the environment,so as to provide new ideas for the application of MN in the disease treatment,diagnosis,and the R&D of detection reagents.