促进动物肠道乳酸杆菌生长的中草药的筛选

Screening of Chinese Herbal Medicines for Promoting Growth of Lactobacillus in Animal Intestine

益生菌对动物健康至关重要,中草药对动物副作用小,筛选出一些能激活益生菌增殖的中草药意义重大。本研究旨在从待筛选的49种中草药中筛选出能激活益生菌乳酸杆菌增殖的目标中草药。将不同的待选中草药制成水提液,以96孔板作为筛选工具,在添加不同中草药水提液前后测定培养液pH差值(△pH)作为中草药是否对乳酸杆菌有激活增殖活性的定性试验,测定乳酸杆菌培养液吸光度差值(△OD值)、平板菌落数作为定量试验,最后以动物试验加以验证,筛选出能激活乳酸杆菌增殖的目标中草药。结果显示:待筛选的49种中草药对乳酸杆菌增殖活性的激活效果各不相同,五味子与山楂表现出较强的激活作用,它们的水提液的最佳作用浓度分别为250和500 mg/mL,白术、木香次之,它们的水提液的最佳作用浓度分别为250和500 mg/mL;汉防己、黄芪等11种中草药再次之,它们均有一定的激活作用;厚朴、大青叶等34种中草药则无激活作用。通过体内和体外试验,本研究筛选出五味子、山楂、白术、木香对乳酸杆菌增殖活性具有较强的激活作用。

Probiotics are essential to animal health.Chinese herbal medicines have little side effects on animals.It is great significance to select some Chinese herbal medicines that can activate probiotics proliferation.The aim of this study was to screen the target Chinese herbal medicines which could activate the proliferation of the probiotic Lactobacillus from 49 Chinese herbal medicines.Different Chinese herbal medicines that to be select-ed were made into water extract.A 96-well plate was used as a screening tool.The pH difference(△pH)in the culture solution was determined before and after added different Chinese herbal medicine water extracts.△pH as a qualitative experiment on whether certain Chinese herbal medicines had activation activity on Lacto-bacillus proliferation.Then,it was used as a quantitative experiment by determining the optical density differ-ence value(△OD value)in the Lactobacillus culture medium and the number of bacterial colony in the plate before and after added different Chinese herbal medicine water extracts.Finally,the Chinese herbal medicine which could activate Lactobacillus proliferation was verified by animal experiment.The results showed that 49 kinds of Chinese herbal medicines to be screened showed different activation activities on Lactobacillus pro-liferation.Fructus schizandrae and Crataegus pinnatifida had the strongest activation effect,and the optimal ac-tion concentrations of their water extracts were 250 and 500 mg/mL,respectively.The activation effect of At-ractylodes macrocephala and Aucklandia lappa was in the second,and the optimal action concentrations of their water extracts were 250 and 500 mg/mL,respectively.There were 11 kinds of Chinese herbal medicines,including Hanfangji and Astragalus membranaceus,were in the third,and they had certain activation effect.Thirty-four kinds of Chinese herbal medicines,including Magnolia officinalis and Daqingye,had no activation effect.Through in vivo and in vitro experiments,Fructus schizandrae,Crataegus pinnatifida,Atractylodes macrocephala and Aucklandia lappa which have a strong activation activity on Lactobacillus proliferation are screened in this study.